[Histonet] Controls with patient specimen on same slide

[Weems, Joyce]

We used to do batch controls too. And we cut unstained slides in a similar fashion also. In order to put the control on the already cut slide, one must stand on one's head or hang themselves upside down from the rafters, but it can be done.. Just put the slide in the water label end first and you have it! Took me years to get it going, and took awhile for techs to adjust, but now it's very routine. And if you use a derm punch you can get lots of control tissue from your blocks without destroying the block. And you save many dollars and give the pathologists assurance that the tissue was treated the same.


Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Richard Cartun
Sent: Tuesday, July 19, 2011 18:40
To: histonet <@t> lists.utsouthwestern.edu; Toni Rathborne
Subject: Re: [Histonet] Controls with patient specimen on same slide

We do not put our positive control tissue on the test slide; we run batch controls.  Many of the unstained slides (breast, GI, and prostate biopsies) that we use for IHC testing are cut in our Histology Laboratory as part of a part-type slide protocol.  For example, we cut 7 slides, 2 sections on each slide, for breast biopsies and stain #1, 4, and 7 with H&E, and then use (if needed) #2, 3, 5, and 6 for IHC.  Therefore, it would be very difficult for us to place the positive control tissue on the same slide.  In addition, I receive a lot of consult cases from other hospitals where they send us unstained slides for testing.  Once again, it would be difficult to place the positive control tissue on the same slide and it would slow us down in terms of starting those slides once they arrive.  However, I think the main reason we don't pursue putting the positive control tissue on the same slide is the fact that it would consume an enormous amount of control tissue.

Richard  

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


>>> "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com> 7/19/2011 
>>> 3:27 PM >>>

Hi,
I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this?  If you're not, why not?
Thanks,
Toni


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