[Histonet] Re: Histonet Digest, Vol 92, Issue 18
Tim Higgins
thiggins <@t> cddmedical.com
Thu Jul 14 09:09:56 CDT 2011
On all stomach biopsies.
Tim
----- Original Message -----
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Sent: Wednesday, July 13, 2011 10:45 PM
Subject: Histonet Digest, Vol 92, Issue 18
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Today's Topics:
1. Quick Drying Mounting Media (Katelin Lester)
2. Re: Quick Drying Mounting Media (Katelin Lester)
3. IHC for H. pylori (Joanne Clark)
4. Re: IHC for H. pylori (Patrick Laurie)
5. QC vs QA (H R)
6. RE: QC vs QA (Amber McKenzie)
7. Re: IHC for H. pylori (Richard Cartun)
8. Re: IHC for H. pylori (Mark Tarango)
9. RELIA Hot Management Job Alert Anatomic Pathology Lab Manager
for top Lab in Austin, TX (Pam Barker)
10. Nbldsu (Paul Scholder)
11. And now, IHC playing cards! (mtitford <@t> aol.com)
12. Re: (Denise Piontek)
13. Question - Anatomic Pathology Manager (Richard Cartun)
14. Tzank Smears (Amy Self)
15. SlideMate Procedure (Laurie Colbert)
16. RE: IHC for H. pylori (Fawn Bomar)
17. Reference labs doing Fast Myosin IHC stain (Sebree Linda A)
18. Re: QC vs QA (SHANE NELSON)
19. Bone Marrow Biopsies (Heather Cooper)
20. out of office (Marilyn.A.Weiss <@t> kp.org)
21. Antigen retrieval survey (Andrea T. Hooper)
22. Re: confocal questions: mixing fluorescent dyes with proteins
(Andrea T. Hooper)
23. Re: In search of a cryostat... (Andrea T. Hooper)
24. Anti-RFP (Andrea T. Hooper)
----------------------------------------------------------------------
Message: 1
Date: Wed, 13 Jul 2011 10:42:25 -0700
From: Katelin Lester <katelin09htl <@t> gmail.com>
Subject: [Histonet] Quick Drying Mounting Media
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CAOEzYbS9j+_CT5UWZ5Hh9CiXT8wEXCyqLDuiNCrYij2=waT6Ag <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi histonet,
We have a brand new Leica CV5030 Coverslipper and I am looking for the
quickest drying media available. We are currently using Micromount by
Surgipath/Leica and it is not drying fast enough.
Any suggestions are appreciated as we have to package our slides for
shipping and the media is either sticking to the bubble wrap or creating
circles on the slides from the pressure of the bubble wrap.
--
Katelin Lester, HTL
Gastroenterology Specialists of Oregon, P.C.
Pathology Laboratory
(971) 224-2408
------------------------------
Message: 2
Date: Wed, 13 Jul 2011 11:14:01 -0700
From: Katelin Lester <katelin09htl <@t> gmail.com>
Subject: Re: [Histonet] Quick Drying Mounting Media
To: Marcia Funk <FUNKM <@t> mercyhealth.com>, Histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CAOEzYbTbRTaiSAPoc9MVL7Mz7ruAwQ2OhQmCbszmvOEytoosWA <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
We are shipping them out same day. At this point, if I can not find a
quicker drying media I will have to delay the shipments by one day.
What temperature do you dry them at?
On Wed, Jul 13, 2011 at 10:50 AM, Marcia Funk <FUNKM <@t> mercyhealth.com> wrote:
> Katelin- do you ship the dame day ? We dry our slides in a oven daily
> and file
> ship the next day . seems to work
> Marcia
>
> Marcia Funk
> Histology Laboratory
> Mercy Medical Center North Iowa
> Mason City, IA, 50401
> 641-428-7907
>
>
> >>> Katelin Lester <katelin09htl <@t> gmail.com> 7/13/2011 12:42 PM >>>
>
> Hi histonet,
> We have a brand new Leica CV5030 Coverslipper and I am looking for the
> quickest drying media available. We are currently using Micromount by
> Surgipath/Leica and it is not drying fast enough.
> Any suggestions are appreciated as we have to package our slides for
> shipping and the media is either sticking to the bubble wrap or creating
> circles on the slides from the pressure of the bubble wrap.
>
>
> --
> Katelin Lester, HTL
> Gastroenterology Specialists of Oregon, P.C.
> Pathology Laboratory
> (971) 224-2408
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
Katelin Lester, HTL
Gastroenterology Specialists of Oregon, P.C.
Pathology Laboratory
(971) 224-2408
------------------------------
Message: 3
Date: Wed, 13 Jul 2011 18:22:17 +0000
From: Joanne Clark <jclark <@t> pcnm.com>
Subject: [Histonet] IHC for H. pylori
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<0494A7D4E8CC254EA2FB81464982E3786E6F61 <@t> S10MAILD001N1.SH10.lan>
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Hi All, was wondering how everyone does their IHC for H. pylori. Do you
automatically run it on all biopsies of the stomach, or do you screen first
with a giemsa and run IHC on the ones that are negative by giemsa? Thanks
for the input!
Joanne Clark, HT
Histology Supervisor
Pathology Consultants of New Mexico
------------------------------
Message: 4
Date: Wed, 13 Jul 2011 11:29:24 -0700
From: Patrick Laurie <foreightl <@t> gmail.com>
Subject: Re: [Histonet] IHC for H. pylori
To: Joanne Clark <jclark <@t> pcnm.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CAKEyg-3G93rn1fSVEJCtY9TZsuAECFkoQhc=NBzb-Ls0Fp0onw <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Our pathology groups decision was to run IHC for H.pylori on all stomach
biopsies.
On Wed, Jul 13, 2011 at 11:22 AM, Joanne Clark <jclark <@t> pcnm.com> wrote:
> Hi All, was wondering how everyone does their IHC for H. pylori. Do you
> automatically run it on all biopsies of the stomach, or do you screen
first
> with a giemsa and run IHC on the ones that are negative by giemsa? Thanks
> for the input!
>
> Joanne Clark, HT
> Histology Supervisor
> Pathology Consultants of New Mexico
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
plaurie <@t> cellnetix.com
------------------------------
Message: 5
Date: Wed, 13 Jul 2011 14:29:30 -0400
From: H R <hrfulklab <@t> gmail.com>
Subject: [Histonet] QC vs QA
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAF1LQBCGaWdTEtvtCiMdmsYxNLmz=2yAMtXkHRiHxeTpcKS36A <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Working on my procedure manual for CLIA and was wondering what the
difference is between QC procedures and QA procedures.?
------------------------------
Message: 6
Date: Wed, 13 Jul 2011 13:32:08 -0500
From: "Amber McKenzie" <amber.mckenzie <@t> gastrodocs.net>
Subject: RE: [Histonet] QC vs QA
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<03C921A1EAF7F541B16543F6EC6A4B370448A5F8 <@t> giamail2.Gia.com>
Content-Type: text/plain; charset="us-ascii"
If you only need to keep QC records for 2 years, is it the same for QA
records?
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of H R
Sent: Wednesday, July 13, 2011 1:30 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] QC vs QA
Working on my procedure manual for CLIA and was wondering what the
difference is between QC procedures and QA procedures.?
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 7
Date: Wed, 13 Jul 2011 14:37:50 -0400
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
Subject: Re: [Histonet] IHC for H. pylori
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, "Joanne Clark" <jclark <@t> pcnm.com>
Message-ID: <4E1DADBE.7400.0077.1 <@t> harthosp.org>
Content-Type: text/plain; charset=US-ASCII
We only run IHC for H. pylori when the appropriate inflammatory background
is present or when the patient has tested positive in the past and we
receive a follow-up gastric specimen where bugs are not identified on H&E.
Running H. pylori IHC on every gastric biopsy is uncalled for and borders on
"Fraud and Abuse".
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax
>>> Joanne Clark <jclark <@t> pcnm.com> 7/13/2011 2:22 PM >>>
Hi All, was wondering how everyone does their IHC for H. pylori. Do you
automatically run it on all biopsies of the stomach, or do you screen first
with a giemsa and run IHC on the ones that are negative by giemsa? Thanks
for the input!
Joanne Clark, HT
Histology Supervisor
Pathology Consultants of New Mexico
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Wed, 13 Jul 2011 11:47:13 -0700
From: Mark Tarango <marktarango <@t> gmail.com>
Subject: Re: [Histonet] IHC for H. pylori
To: Richard Cartun <Rcartun <@t> harthosp.org>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, Joanne Clark <jclark <@t> pcnm.com>
Message-ID:
<CACJwj1+Qqsw9meiUzkcnS720A59LQcz7wKazsdAiCffzYi1NJg <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
We run the H. pylori IHC stain on all stomach specimens to improve turn
around time; however, when an inflammatory background is not appreciated by
the pathologist the charge is removed before sign-out and we eat the cost of
producing the slide.
Mark
On Wed, Jul 13, 2011 at 11:37 AM, Richard Cartun
<Rcartun <@t> harthosp.org>wrote:
> We only run IHC for H. pylori when the appropriate inflammatory background
> is present or when the patient has tested positive in the past and we
> receive a follow-up gastric specimen where bugs are not identified on H&E.
>
> Running H. pylori IHC on every gastric biopsy is uncalled for and borders
> on "Fraud and Abuse".
>
> Richard
>
> Richard W. Cartun, MS, PhD
> Director, Histology & Immunopathology
> Director, Biospecimen Collection Programs
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 545-1596 Office
> (860) 545-2204 Fax
>
>
> >>> Joanne Clark <jclark <@t> pcnm.com> 7/13/2011 2:22 PM >>>
> Hi All, was wondering how everyone does their IHC for H. pylori. Do you
> automatically run it on all biopsies of the stomach, or do you screen
first
> with a giemsa and run IHC on the ones that are negative by giemsa? Thanks
> for the input!
>
> Joanne Clark, HT
> Histology Supervisor
> Pathology Consultants of New Mexico
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 9
Date: Wed, 13 Jul 2011 15:11:29 -0400
From: "Pam Barker" <relia1 <@t> earthlink.net>
Subject: [Histonet] RELIA Hot Management Job Alert Anatomic Pathology
Lab Manager for top Lab in Austin, TX
To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <756593A6AD9F49EFA099D740BFD653AB <@t> ownerf1abaad51>
Content-Type: text/plain; charset="iso-8859-1"
Hi Histonetters!!
Here is a an exciting opportunity HOT OFF THE PRESSES!
Anatomic Laboratory Manager - Leading Diagnostic Lab - Austin, TX
RELIA Solutions the nation's only recruiting firm specializing in the
permanent placement of histology professionals has been engaged by a
leading Pathology Lab in Austin, TX to assist in their search for an
Anatomic Pathology Laboratory Manager. My client offers a very
competitive compensation package including bonuses and relocation
assistance along with an incredible benefits package.
This person will be responsible for the overall daily and financial
operation of the Histology and Cytology Departments. Direct supervision
of their department supervisors, delegating responsibilities as
appropriate to these General Supervisors. Working directly in support
of the Technical supervisors for these areas. As well as developing or
approving department specific policies. Participating in the
development and approval of laboratory wide policies and procedures;
ensuring that all laboratory policies and procedures are followed;
accepting and supporting activities delegated by the Medical Director
and Vice President of Operations.
Qualifications:
1.Bachelors or equivalent degree in the sciences.
2.Minimum 10 years experience in laboratory operations.
3.ASCP certification or equivalent for testing areas.
4.Leadership skills and supervisory abilities.
5.Organize and coordinate diverse activities.
6.Continuous improvement experience
For more information contact Pam Barker at relia1 <@t> earthlink.net or toll
free at 866-607-3542.
Keywords histology histologist anatomic pathology cytology
Thank You!
Pam Barker
President
RELIA
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: relia1 <@t> earthlink.net
www.facebook.com search Pam Barker RELIA
www.linkedin.com/reliasolutions
www.myspace.com/pamatrelia
www.twitter.com/pamatrelia
------------------------------
Message: 10
Date: Wed, 13 Jul 2011 14:14:18 -0500
From: Paul Scholder <pauls <@t> ncimicro.com>
Subject: [Histonet] Nbldsu
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CA+m6khkewS8rvf8ib+E9-Adm-wRYaBVTWz3A4A2RfuZ-EKzaEw <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
------------------------------
Message: 11
Date: Wed, 13 Jul 2011 15:18:55 -0400
From: mtitford <@t> aol.com
Subject: [Histonet] And now, IHC playing cards!
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <8CE0FA8D804645B-2794-3F7C <@t> webmail-d180.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"
Those of you that like freebies (like Dako mousepads, Thermo pens and Sigma
coffee mugs) will be amused to learn that Cell Marque are giving away packs
of playing cards. Each card shows a section stained with a different
antobody (one of theirs of course, together with the catalogue number).
No, I don't have shares in Cell Marque, but I am continually amused by the
different methods companies in all walks of life use to advertise their
products. Some are, well, just original!
Michael Titford
Pathology USA
Mobile AL USA
------------------------------
Message: 12
Date: Wed, 13 Jul 2011 15:23:32 -0400
From: Denise Piontek <dbpiontek <@t> hotmail.com>
Subject: [Histonet] Re:
To: <camille_diges <@t> bio-rad.com>, <cdiges <@t> gmail.com>,
<canvaspeople <@t> email.canvaspeople.com>, <dpantano <@t> hrecruiters.com>,
<deedra711 <@t> yahoo.com>, <histonet <@t> lists.utsouthwestern.edu>,
<info <@t> littlepeopleplayhouse.com>
Message-ID: <COL112-W1130C298CB68D864D1FBC7AB470 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
It's the most powerful treatment I've ever used!...
http://israelnlp.com/inbox.php?epyahooID=76n4
------------------------------
Message: 13
Date: Wed, 13 Jul 2011 15:27:55 -0400
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
Subject: [Histonet] Question - Anatomic Pathology Manager
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4E1DB97B.7400.0077.1 <@t> harthosp.org>
Content-Type: text/plain; charset=US-ASCII
How many Hospital-based Pathology Departments with 55,000 surgical
specimens, 100,000 PAPs, and 6,000 non-GYN cytology cases have separate
managers for Cytology and Histology? Thank you.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax
------------------------------
Message: 14
Date: Wed, 13 Jul 2011 15:42:38 -0400
From: Amy Self <ASelf <@t> georgetownhospitalsystem.org>
Subject: [Histonet] Tzank Smears
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<EEED2FF216617D45AFF64BF53EC4F9C712BE04A614 <@t> GMHDTCEXCH.gmhpost.com>
Content-Type: text/plain; charset="us-ascii"
Hello Histonetters,
I was wandering if anyone had a SOP for the collection and
processing/staining of tzank smears that they would be willing to share with
me?
Thanks in advance for everyone's help.
Amy Self
Georgetown Hospital System
Dept. of Pathology
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Thank you.
------------------------------
Message: 15
Date: Wed, 13 Jul 2011 12:46:31 -0700
From: "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com>
Subject: [Histonet] SlideMate Procedure
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<57BE698966D5C54EAE8612E8941D76830AD2AE7A <@t> EXCHANGE3.huntingtonhospital.com>
Content-Type: text/plain; charset="us-ascii"
Does anyone have a PSLIM/SlideMate procedure that they would be willing
to share with me?
Laurie Colbert
------------------------------
Message: 16
Date: Wed, 13 Jul 2011 16:07:01 -0400
From: Fawn Bomar <Fawn.Bomar <@t> HalifaxRegional.com>
Subject: RE: [Histonet] IHC for H. pylori
To: Patrick Laurie <foreightl <@t> gmail.com>, Joanne Clark
<jclark <@t> pcnm.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E779667A9118F74D8B7D82A1891BE030E44797E875 <@t> exch-2k7.hrhs.com>
Content-Type: text/plain; charset="us-ascii"
We run giemsa's on all stomach bx's
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu
[histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patrick Laurie
[foreightl <@t> gmail.com]
Sent: Wednesday, July 13, 2011 2:29 PM
To: Joanne Clark
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC for H. pylori
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Date: Wed, 13 Jul 2011 11:29:24 -0700
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From: Patrick Laurie <foreightl <@t> gmail.com>
To: Joanne Clark <jclark <@t> pcnm.com>
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Subject: Re: [Histonet] IHC for H. pylori
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Our pathology groups decision was to run IHC for H.pylori on all stomach
biopsies.
On Wed, Jul 13, 2011 at 11:22 AM, Joanne Clark <jclark <@t> pcnm.com> wrote:
> Hi All, was wondering how everyone does their IHC for H. pylori. Do you
> automatically run it on all biopsies of the stomach, or do you screen
first
> with a giemsa and run IHC on the ones that are negative by giemsa? Thanks
> for the input!
>
> Joanne Clark, HT
> Histology Supervisor
> Pathology Consultants of New Mexico
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
plaurie <@t> cellnetix.com
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Message: 17
Date: Wed, 13 Jul 2011 15:27:48 -0500
From: "Sebree Linda A" <LSebree <@t> uwhealth.org>
Subject: [Histonet] Reference labs doing Fast Myosin IHC stain
To: <Histonet <@t> lists.utsouthwestern.edu>
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I'm looking for a reference lab that can do Fast Myosin
immunohistochemistry staining on FFPE human tissue.
Thanks,
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
------------------------------
Message: 18
Date: Wed, 13 Jul 2011 13:38:17 -0700 (PDT)
From: SHANE NELSON <nelsonrnch <@t> verizon.net>
Subject: Re: [Histonet] QC vs QA
To: Histonet <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <1310589497.49973.YahooMailRC <@t> web84308.mail.re1.yahoo.com>
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Quality Assurance outlines all required QC activities used to identify
policies
or procedures that are not working as desired, especially those that are not
immediately apparent from following routine QC procedures.
The purspose of a QA plan is effectively achieved by providing a mechanism
for
identifying problems so that corrective actions can be taken to ensure,
reliable, and prompt reporting of patient test results.
Quality Control is to assess the effectiveness of the quality control system
to
identify inaccuracies in test systems prior to result reporting. To verify
the proper use and evaluation of quality control data by testing personnel.
To
assess test methodologies based upon past QC results.
THANK YOU,
PATTI RUBEN-NELSON H.T.(ASCP)
PNP LABORATORY CONSULTANTS
SUPERVISOR/DGC
P.O. BOX 412
CABAZON, CA. 92230
cell (909) 841-9761
nelsonrnch <@t> verizon.net
________________________________
From: H R <hrfulklab <@t> gmail.com>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Wed, July 13, 2011 11:29:30 AM
Subject: [Histonet] QC vs QA
Working on my procedure manual for CLIA and was wondering what the
difference is between QC procedures and QA procedures.?
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------------------------------
Message: 19
Date: Wed, 13 Jul 2011 13:41:46 -0700 (PDT)
From: Heather Cooper <hctrupath <@t> att.net>
Subject: [Histonet] Bone Marrow Biopsies
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1310589706.46384.YahooMailRC <@t> web80013.mail.sp1.yahoo.com>
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What is the procedure for collecting and processing bone marrow bx? How much
time should it be placed in DECAL??????????????
------------------------------
Message: 20
Date: Wed, 13 Jul 2011 16:02:55 -0700
From: Marilyn.A.Weiss <@t> kp.org
Subject: [Histonet] out of office
To: histonet <@t> lists.utsouthwestern.edu
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<OF2B401E0E.D37A61EF-ON882578CC.007E9C49-882578CC.007E9C4A <@t> kp.org>
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I will be out of the office starting 07/13/2011 and will not return until
07/18/2011.
In my absence please ask for Mary . If this is urgent or you need to speak
to me directly you can contact me on my cell phone number 858-472-4266. If
it concerns a Mohs to be scheduled you can e-mail me or call on my cell.
Thank you.
------------------------------
Message: 21
Date: Wed, 13 Jul 2011 20:10:16 -0700 (PDT)
From: "Andrea T. Hooper" <andreahooper <@t> rocketmail.com>
Subject: [Histonet] Antigen retrieval survey
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
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<1310613016.98546.YahooMailClassic <@t> web113107.mail.gq1.yahoo.com>
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Hi All,
I am doing a survey and will be happy to compile results and share if folks
will respond! What is your favorite antigen retrieval method and/or panel?
Buffer
Source/composition
Temperature
Device
Thanks, Andrea
------------------------------
Message: 22
Date: Wed, 13 Jul 2011 20:13:43 -0700 (PDT)
From: "Andrea T. Hooper" <andreahooper <@t> rocketmail.com>
Subject: Re: [Histonet] confocal questions: mixing fluorescent dyes
with proteins
To: tgenade <@t> gmail.com
Cc: Histonet <histonet <@t> lists.utsouthwestern.edu>
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<1310613223.80664.YahooMailClassic <@t> web113107.mail.gq1.yahoo.com>
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It will depend on how your scope is configured. On our system, when using
the three you have outlined I routinely use CY5 or Alexa633 (or equivalent
in far red category) on our scope. It's actually my personal favorite. If
you have any doubts check out the spectral viewer on Invitrogen and also BD
Bioscience's website.
--- On Wed, 7/13/11, Tyrone Genade <tgenade <@t> gmail.com> wrote:
From: Tyrone Genade <tgenade <@t> gmail.com>
Subject: [Histonet] confocal questions: mixing fluorescent dyes with
proteins
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Date: Wednesday, July 13, 2011, 11:23 AM
Hello,
I'm working on a Zeiss confocal microscope. We use chiefly Alexa488
and Cy3 together with Hoechst 33258. Can anyone suggest a fluorescent
protein which can be discerned amongst the Alexa488, Cy3 and Hoechst
33258 emission spectra? I was thinking maybe mBanana?
Thanks
--
Tyrone Genade
email: tgenade <@t> gmail.com
tel: +27-84-632-1925 (c)
Department of Human Biology
University of Cape Town
South Africa
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------------------------------
Message: 23
Date: Wed, 13 Jul 2011 20:17:03 -0700 (PDT)
From: "Andrea T. Hooper" <andreahooper <@t> rocketmail.com>
Subject: Re: [Histonet] In search of a cryostat...
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, Tom McNemar
<TMcNemar <@t> lmhealth.org>, Rene J Buesa <rjbuesa <@t> yahoo.com>
Message-ID:
<1310613423.83362.YahooMailClassic <@t> web113106.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Agree, switching to Leica with disposable blade holder changed my life.
Never looked back. Love the CM3050S.
--- On Sun, 7/10/11, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] In search of a cryostat...
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>,
"Tom McNemar" <TMcNemar <@t> lmhealth.org>
Date: Sunday, July 10, 2011, 3:55 PM
Buy Leica
René J.
--- On Sun, 7/10/11, Tom McNemar <TMcNemar <@t> lmhealth.org> wrote:
From: Tom McNemar <TMcNemar <@t> lmhealth.org>
Subject: [Histonet] In search of a cryostat...
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Sunday, July 10, 2011, 10:53 AM
Hello all,
I am looking to budget for a new cryostat and was wondering what others are
using. I would like one that is safe, well lit, roomy, and has some sort of
on-board decontamination routine. All input appreciated. Thanks in advance.
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org<mailto:tmcnemar <@t> lmhealth.org>
www.LMHealth.org<file:///C:\Documents%20and%20Settings\TMCNEMAR\Application%
20Data\Microsoft\Signatures\www.LMHealth.org>
________________________________
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------------------------------
Message: 24
Date: Wed, 13 Jul 2011 20:40:16 -0700 (PDT)
From: "Andrea T. Hooper" <andreahooper <@t> rocketmail.com>
Subject: [Histonet] Anti-RFP
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
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<1310614816.57254.YahooMailClassic <@t> web113116.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Is anyone currently staining for RFP in FFPE or frozen sections using a
detecting antibody? I am curious as to what anti-RFP Ab would be
recommended. There is one I plan to try from Invitrogen (rabbit anti-RFP)
but of course would love feedback if people have used one in particular.
Thanks, Andrea
------------------------------
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