[Histonet] pan B-cell marker for frozen mouse tissue

[Adam .]

B220 is a pretty good B-cell marker, although you're right that it's not
restricted to the B-cell lineage. It's also expressed in some T-cells and NK
cells, for example. Cells that are double positive for B220 and IgG or IgM
are B-cells almost exclusively. CD19 is a good marker for mature B-cells,
although pre-pro B cells do not express CD19. If you're looking at any organ
other than bone marrow or spleen, there shouldn't be many pre-pro B cells
and you can probably just use CD19.

Adam

On Mon, Jul 11, 2011 at 1:37 PM, Michele Wich <mwich <@t> 7thwavelabs.com> wrote:

> I am trying to find the most appropriate pan B-cell marker for IHC on
> frozen mouse tissue. I know that CD45R/B220 used to be the most commonly
> used, but recent publications suggest PAX5 or CD19 as more restricted to
> the B-cell lineage. Does anyone have any other suggestions, or are these
> generally what people are using these days?
>
> Thanks for any info.
>
>
>
>
>
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