Fw: [Histonet] Re: Embedding Media

[Tim Higgins]

> Last comment on the paraffin conversion for me.
>
> You can use bees wax if you want in your processor and embedder that
doesn't
> mean you going to get the optimal results for every situation.  Paraffin
> types are a personal preference for most people or labs.  It's a little
more
> hassle to keep two paraffins on hand instead of just one..  Low polymer
> paraffins penetrate better, faster, so and so forth and the higher polymer
> paraffins take a little longer but supports tissue better for sectioning.
> There are positives and negatives for both.  Using one paraffin type is
> fine, all personal preference.  We could go on forever with this!!
>
>
> I am not endorsing any paraffin on here, used many different paraffins and
> each work as anticipated.
>
> Thanks,
>
> Tim
>
>
>
>
> Sent: Monday, July 11, 2011 5:44 AM
> Subject: Re: [Histonet] Re: Embedding Media
>
>
> Amita,
>
> The paraffin that we use is also a Leica product. It was formerly sold
under
> the Surgipath label, but since to companies have merged together, it now
> sells under the Leica name. It is a "beaded" pellet paraffin and has a
> version suitable for infiltration and a version suitable for embedding.
The
> box of the embedding paraffin suggests it can be utilized for *both*
> purposes, however we do not choose to use it that way.  So yes, we use two
> different types of paraffin.
>
> Hope that helps!
> Michelle
>
>
> > I continuation of same discussion , i would like to have clarification
for
> > our lab..we use ....beaded paraffin "Leica Paraplast"  both for
processing
> > and embedding . Do you advice us to use different paraffin for both
steps?
> >
> >
> > Paula, Please share if you get any feed back on Richard Allan product.
> >
> > Amita
> >
> >
> > > Subject:        [Histonet] Re: Embedding Media
> > Sent by:        histonet-bounces <@t> lists.utsouthwestern.edu
> >
> >
> >
> > Hi Paula,
> >
> > Obviously there are no "compression free" paraffin's on the market.
Look
> > for a paraffin with a higher polymer content, this makes the paraffin
> > firmer
> > and less likely to compress.  Other factor for into section compression
> > but
> > paraffin is a good place to start.
> >
> > The higher polymer paraffin's are not as suited for processing, it is
more
> > for the embedding portion of the process.
> >
> > Use a paraffin with lower polymers content in the processor and higher
for
> > embedding and you will like the results.
> >
> >
> > Tim
> >
> >
> > > Subject: [Histonet] Embedding Media
> > To: <histonet <@t> lists.utsouthwestern.edu>
> > >
> > Hello,
> >
> >
> >
> > We are considering a switch to a different brand of paraffin and this is
> > because I feel we are having too many compressions in some of our tissue
> > sections.  Currently, we use Tissue Path Paraplast, regular.
> >
> >
> >
> > I would like to get feedback from you as to what you prefer.  Looking on
> > line, Richard Allan has a product called Signature Series Paraffin that
> > comes in a type L that offers compression-free sections and I was also
> > hoping to get any feedback on that product.
> >
> >
> >
> > I would greatly appreciate any suggestions and thoughts.
> >
> > Thanks in advance,
> >
> > Paula
> >
> >
> >
>