[Histonet] Direct Mouse IgG

[Ross Benik]

Hello Histonet,

Does anyone have a working protocol that stains directly for mouse IgG (fluorescent) on frozen sections?  We are currently staining with a goat anti mouse IgG 555 (highly cross absorbed) and we have having a hard time deciding what is specific staining and what is no.  Information such as micron thickness, method of fixation (acetone/methanol, NBF etc) and staining parameters would be great!

Thanks,
Ross