[Histonet] Routine H & E stain,

[amitapandey <@t> torrentpharma.com]

We are using Xylene sulphur free- LR grade( from Rankem, India). I don't 
know about its synthetic nature but i will inquire this to manufacture.
Mountant DPX from Merck .

Do you have any suggestion on this combination?

Thanks for all your feed back.

Amita



From:   <sdysart <@t> mirnarx.com>
To:     <amitapandey <@t> torrentpharma.com>
Cc:     <histonet <@t> lists.utsouthwestern.edu>
Date:   07/07/11 07:10 PM
Subject:        RE: [Histonet] Routine H & E stain,



I have always used Harris and have not experienced this problem?  I do
buy mine pre-made from VWR (cat#-95057-858).  I agree with the xylene
comment, also what mounting media are you using?  The mounting media can
fade your stain over time as well. 
Good Luck!

Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
amitapandey <@t> torrentpharma.com
Sent: Wednesday, July 06, 2011 11:23 PM
To: Histonet; histonet-bounces <@t> lists.utsouthwestern.edu; histonet
Subject: [Histonet] Routine H & E stain,

Hello Histonetters,

I am from toxicopathology lab where we perform H&E on rat tissues and 
store these slides for 10 long years.

We use self lab prepare Harris hematoxylin (Hematoxylin crystal+Alcohol+

Ammonium alum +D/W and Mercuric oxide for ripening) and 1% aqueous
eosin. 
The staining result is good (Purplish pink look stained slide) on all 
tissue, but  our observation is after 5-6 months time this get faded and

become towards pinkish type though we can observe the slide. 
We want to retain its fresh stained color. 

Please suggest me how to keep stable stain for longer period or do you 
suggest to switch to any other type of haematoxylin - prepared or 
commercially available?

Looking forward for your feed back.
Amita
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