[Histonet] Routine H & E stain,

[SHANE NELSON]

Are you using synthetic xylene in your H&E/COVER SLIPPING protocol. If yes, you 
might want to consider ending your protocol with the real thing (XYLENE). I had 
the same situation until we switched to real XYLENE. No more H&E fading.




THANK YOU,
 
PATTI RUBEN-NELSON  H.T.(ASCP) 
PNP LABORATORY CONSULTANTS
SUPERVISOR/DGC
P.O. BOX 412
CABAZON, CA. 92230
cell (909) 841-9761 
nelsonrnch <@t> verizon.net




________________________________
From: "amitapandey <@t> torrentpharma.com" <amitapandey <@t> torrentpharma.com>
To: Histonet <histonet <@t> lists.utsouthwestern.edu>; 
histonet-bounces <@t> lists.utsouthwestern.edu; histonet 
<histonet-request <@t> lists.utsouthwestern.edu>
Sent: Wed, July 6, 2011 9:22:38 PM
Subject: [Histonet] Routine H & E stain,

Hello Histonetters,

I am from toxicopathology lab where we perform H&E on rat tissues and 
store these slides for 10 long years.

We use self lab prepare Harris hematoxylin (Hematoxylin crystal+Alcohol+ 
Ammonium alum +D/W and Mercuric oxide for ripening) and 1% aqueous eosin. 
The staining result is good (Purplish pink look stained slide) on all 
tissue, but  our observation is after 5-6 months time this get faded and 
become towards pinkish type though we can observe the slide. 
We want to retain its fresh stained color. 

Please suggest me how to keep stable stain for longer period or do you 
suggest to switch to any other type of haematoxylin - prepared or 
commercially available?

Looking forward for your feed back.
Amita
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