[Histonet] Leaving sample over night in ETOH 70% during fixation

[Itai Moshe]

Dear All,
Does leaving the samples (diaphragm, liver) over night in ethanol 70% at 4C
during the fixation process, will be better for the tissue fixation, and
does not harm the sample ?
Does the fixation process should be done straight forward from the first
step to the last one without any over night stops (except from the PFA,
Bouin's step) ?


My fixation protocol is like this:
1) immediately after killing the mouse i'm putting the sections in a
fixation solution that is made from: 10ml formaldehyde 37%+5ml PBSx20+85ml
DDW  - pH 7, Or bouin's solution over night at 4C.
2) ETOH 70%, ETOH 80%, ETOH 96%, ETOH 100% x2 - each for 1Hr at RT
3) Xylen x2 - each for 1Hr at RT.
4) Paraffin x3 - each at 60C for 1Hr.

Thank you all very much in advance

Itai M
Doe