[Histonet] Processing

Laurie Colbert laurie.colbert <@t> huntingtonhospital.com
Wed Jul 6 16:31:38 CDT 2011


We heat from 38-40 degrees for all reagents except the paraffin, which
is heated to 60 degrees.  I don't use any heat at all on my small
biopsies.

Laurie Colbert

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Nicole
Schuster
Sent: Wednesday, July 06, 2011 12:58 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Processing

For large tissue specimens (prostate, placenta, etc.) what temperature
do you use for the solutions on your processor? We currently have them
all set at 40 degrees Celsius. For paraffin it is set at 60 degrees
Celsius. I read the dehydrating and clearing solutions should be at
ambient temperatures... Thought I'd check into it. Thanks! :)

Sent from my iPhone
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