[Histonet] RE: ...maybe a little more specific

Rene J Buesa rjbuesa <@t> yahoo.com
Fri Jul 1 14:58:06 CDT 2011


Sarah:
Ronald's suggestion of using Ulex europaeus lectin is a great idea. I second it.
René J.

--- On Fri, 7/1/11, Houston, Ronald <Ronald.Houston <@t> nationwidechildrens.org> wrote:


From: Houston, Ronald <Ronald.Houston <@t> nationwidechildrens.org>
Subject: [Histonet] RE: ...maybe a little more specific
To: "'sgoebel <@t> mirnarx.com'" <sgoebel <@t> mirnarx.com>, "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Friday, July 1, 2011, 2:10 PM


Sarah, 
if you don't want to go to the hassle of working up antibodies on xenografts, you could use Ulex Europaeus lectin histochemistry which should work on any species - great for capillaries

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of sgoebel <@t> mirnarx.com
Sent: Friday, July 01, 2011 2:03 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] ...maybe a little more specific

So am getting lots of responses for IHC stains (which is ok), but I am
doing these stains on mouse xenografts and the background is getting in
my way a lot.  Was just trying to find a simple special that would do
the trick =)

If IHC is the only way, so be it...was just hoping...



What about Verhoeff???



Sarah Goebel-Dysart, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912



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