[Histonet] fixation question for IHC

Margaret Blount mab70 <@t> medschl.cam.ac.uk
Mon Jan 31 03:00:36 CST 2011

It is possible to freeze paraformaldehyde in aliquots, so you don't have
to keep on making it up repeatedly as we did in the old days. I have
also seen that you can buy Pure formaldehyde, but I don't remember who
the supplier was. It was in sealed ampoules and was 16% so you could
dilute it in your preferred buffer. I have to say that this was about 10
years ago!


Miss Margaret Blount
Histology Manager
Metabolic Research Laboratories
Level 4 Institute of Metabolic Science
Box 289, Addenbrooke's Hospital
Hills Road, Cambridge, CB2 0QQ

Tel 01223 769061/336079

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jan
Sent: 28 January 2011 18:15
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] fixation question for IHC

Is there a difference between using paraformaldehyde and neutral
buffered formalin when choosing a fixative for IHC?  I would prefer to
use formalin because of easier preparation, but am willing to put in the
extra time to make fresh paraformaldehyde solution if there is a
compelling reason.

Jan Berry
University of Michigan
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