[Histonet] glass cleaner

Akemi Allison akemiat3377 <@t> yahoo.com
Wed Jan 26 14:15:29 CST 2011



Alternative to Acid Cleaning Glassware

5% Clorox is a very inexpensive and readily available chemical  
treatment solution, which is an alternative to acid cleaning.  All  
glassware used for special stains MUST be cleaned in the following  
manner.  This method MUST be used when using glassware for special  
stains sensitive to metal, such as FE, GMS, Retic, Jones, Dieterle,  
Steiner and Warthin-Starry.
1.            Make fresh each morning a 5% Clorox solution containing  
1/2 Tbsp. per 5 gallons. of Liqui-Nox.

2.            Soak all glassware in this solution for a minimum of 30  
minutes to remove any stains, and then scrub with brush until all  
residue is removed.

3.            For hard to get out stains, soak in a stronger solution  
of Clorox.

4.            Rinse with copious amounts of tap water, followed by  
several changes of deionized water.  All traces of Clorox must be  
5.            Place glassware on drying rack or upside down to dry  

6.            Use caution that NO metal instruments touch glassware.   
This will contaminate certain solutions, which must be free of any  
metal, such as Iron and Silver Stains.

7.            After glassware is dry, put parafilm or gauze around  
top so no airborne contaminate may enter.
8.            Put glassware away.

Akemi Allison BS, HT (ASCP) HTL
Phoenix Lab Consulting
Tele: 408.335.9994
E-Mail: akemiat3377 <@t> yahoo.com

On Jan 26, 2011, at 11:42 AM, Johnson, Nacaela wrote:

> Hello....
> I am looking for suggestions on the best chemical cleaner for  
> glassware
> that is used for special stains.  Any ideas?
> Thanks,
> Nacaela Johnson
> Histology Technician
> KCCC Pathology
> 12000 110th St., Ste. 400
> Overland Park, KS 66210
> Office:  913-234-0576
> Fax:  913-433-7639
> Email:  Nacaela.Johnson <@t> USOncology.com
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