[Histonet] FX111a Protocol

Setlak, Lisa LSetlak <@t> childrensmemorial.org
Wed Jan 26 10:56:51 CST 2011

We run FactorX111a clone AC-1A1 on the Ultra, here is our protocol:
1. De-paraffinazation (selected)
2. Cell conditioning #1 20 minutes, then 36 minutes.
3. Antibody for 24 minutes
4. Ultra wash (selected)
5. Hematoxylin 4 minutes
6. Bluing 4 minutes.

Hope this helps.
Lisa V.
Children's Memorial Hospital
Chicago, IL 60616

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sheila Fonner
Sent: Wednesday, January 26, 2011 8:46 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] FX111a Protocol

Does anyone have a current protocol for Factor X111a on the Ventana Ultra
that is working for you?  I would appreciate any info or support you could
give.  We are using the AC-1A1 clone from Cell Marque for derms.  Thanks!



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