[Histonet] cutting replacement patches

Tue Jan 25 14:22:03 CST 2011

Hi All,

Does anyone know a company that sells cutting patches?  The ones we use are black with an adhesive back that "sticks" to our cutting board.  Seems like the company we were getting them from no longer carries them..

Thanks for your help
Myra Huth,HT(ASCP)
Bay Medical Center
Pathology Dept.
Panama City, Florida

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From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Tuesday, January 25, 2011 12:04 PM
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Subject: Histonet Digest, Vol 86, Issue 33

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Today's Topics:

   1. re: storing acetic acid (Smith, Allen)
   2. Re: Banana smelling chemical in our formalin (Robert Richmond)
   3. Re: gram stain (Robert Richmond)
   4. RE: g ratio (Houston, Ronald)

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Message: 1
Date: Tue, 25 Jan 2011 16:34:52 +0000
From: "Smith, Allen" <asmith <@t> mail.barry.edu>
Subject: [Histonet] re: storing acetic acid
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<AEE9D3B1BA593A49B23DA5A6A375654C188A26C7 <@t> EX2010-02.barrynet.barry.edu>

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We have separate storage cupboards for inorganic acids (many of which are oxidizing agents) and organic acids (many of which are flammable).  If we didn't have an organic acids cupboard, we would store it with organic solvents.  The reaction of acetic acid with nitric acid might be violent, the reaction of acetic acid with ethanol is slow and quiet.

> -Allen A. Smith

>  Professor of Anatomy

>  Barry University School of Podiatric Medicine

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Message: 2
Date: Tue, 25 Jan 2011 11:31:49 -0500
From: Robert Richmond <rsrichmond <@t> gmail.com>
Subject: [Histonet] Re: Banana smelling chemical in our formalin
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<AANLkTi=sK+zhJ-K8-CiCkozrdD3cYBOksLZj3muszcSg <@t> mail.gmail.com>
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Amyl acetate ("banana oil") is produced by an alternate fermentation
pathway by some of the yeasts used to make Hefeweizen type beers. The
thought of recycling such a fine beer horrifies me.

Ethyl acetate, formed by esterification of ethanol with acetic acid as
fixatives such as Davidson's and O-Fix age, smells like airplane dope
(if you're old enough to remember airplane dope) or nail polish
remover (if you aren't). Obviously these fixatives could easily be
mixed with formalin for recycling, since they also contain
formaldehyde.

About 25 years ago there was a fad for "odor masked" formaldehyde
solutions. The ones I used smelled even worse than formaldehyde, but
they didn't smell like banana oil. I don't know if these solutions are
still available.

Bob Richmond
Samurai Pathologist
Knoxville TN

------------------------------

Message: 3
Date: Tue, 25 Jan 2011 11:49:57 -0500
From: Robert Richmond <rsrichmond <@t> gmail.com>
Subject: [Histonet] Re: gram stain
To: histonet <@t> lists.utsouthwestern.edu
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	<AANLkTin2tiuHuyx8v2OMi_hWa4Fd5LqtioZbj7rFFzB8 <@t> mail.gmail.com>
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The original Brown-Hopps tissue gram stain was published in Lee Luna's
AFIP Manual, 3rd ed. 1968. It did not require picric acid, ethyl
ether, or acetone. I remember that the stain was widely used after
that.

The AFIP manual method does require ethylene glycol monoethyl ether
(Cellosolve 1), which is less of a fire hazard than is ethyl ether.
See the MSDS at
http://www.jtbaker.com/msds/englishhtml/e2600.htm
before ordering it, though.

Freida Carson (in the 2nd edition of her book - I don't have the 3rd)
restored the picric acid and acetone in her modification of the
Brown-Hopps method.

Putting on my pathologist hat now - tissue gram stains are greatly
overrated - they don't work nearly as well as they do on smears. If
you want to see or count bacteria in tissue sections, use a simple
blue stain (toluidine blue, or Diff-Quik II or its generic
equivalent).

Bob Richmond
Samurai Pathologist
Knoxville TN

------------------------------

Message: 4
Date: Tue, 25 Jan 2011 12:13:16 -0500
From: "Houston, Ronald" <Ronald.Houston <@t> nationwidechildrens.org>
Subject: [Histonet] RE: g ratio
To: 'Bataillon Michel' <Michel.Bataillon <@t> citox.com>,
	"histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<E02E1309B208F94C83B968E45781001A234C53BCCD <@t> NCHEXMBX01.columbuschildrens.net>

Content-Type: text/plain;	charset="iso-8859-1"

Michel,

Most studies involving morphometric studies of peripheral nerves involve "thick" plastic sections stained with toluidine blue. In my opinion, you are unlikely to get consistent and acceptable staining results on paraffin sections for the simple reason that the intense blue staining on thick EM sections is more from the osmium tetroxide than the toluidine blue per se.

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager

ChildLab, a Division of Nationwide Children's Hospital

www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.houston <@t> nationwidechildrens.org<mailto:ronald.houston <@t> nationwidechildrens.org>
www.NationwideChildrens.org<http://www.NationwideChildrens.org>

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bataillon Michel
Sent: Tuesday, January 25, 2011 5:16 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] g ratio

Hello,

We have to measure the "g ratio" (ratio of the inner axonal diameter to the total outer diameter) - rat sciatic nerve - at least 500 myelinated fibers per nerve will be evaluated with a standard imaging soflware.

Do you think that we can perform this work with thin paraffin slides - 1.5µm (stained with toluidine blue), in spite of the best practice seems to prepare semithin sections from epoxy resin blocks.

Thank you,

Michel

Michel BATAILLON

Histology

Evreux, France

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