[Histonet] NEW CAP?

Kuhnla, Melissa Melissa.Kuhnla <@t> chsli.org
Fri Jan 21 06:52:12 CST 2011

I am not a CAP inspected lab, but I do QC my ventana detection kits in
two different ways.  They are both very simple and manual. Both came to
be because of faulty detections kits and actually events we experienced.
1. Load the detection kit on a wedge and manually dispense each
dispenser onto a paper towel. Visually inspect the drops sizes. They all
should be equal in size. Throughout the life of the dispenser I also
randomly check visually the levels in each of the components. They
should deplete at the same rate.
2. Dispense the first three dispensers in the same spot on the paper
towel (the inhibitor, multimer, and chromogen).  The wet make on the
towel should turn brown proving that the chemical reaction will take

I know it seems kinda silly, but this has actually saved us in the past
from using a defective kit.

I am curious to hear if anyone is actually slides.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Behnaz
Sent: Thursday, January 20, 2011 12:49 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] NEW CAP?

New lots of antibody and detection system reagents are tested in
parallel with old lots.
Record of validation of new reagents/shipments
I was wondering if you are using Ventana xt, How are you testing each
time you use new DAB-kit ( new lot#)
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