[Histonet] RE: Myocardial Biopsies

Weems, Joyce JWeems <@t> sjha.org
Thu Jan 20 11:16:45 CST 2011

We've done them for years...
	6 levels on 2 slides - L1-L3, L4-L6  1st level placed near label end of the slide No unstained.
	We do C4d when ordered. 

For cases with no previous biopsies - Pick up unstained slides at level 3 for: 
				PAS, Masson Trichrome, Iron, Congo Red and Sulfonated Alcian Blue

Best, j

Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jeffery.Miller <@t> spectrum-health.org
Sent: Thursday, January 20, 2011 12:06
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Myocardial Biopsies

My hospital has recently started performing heart transplants and we are now receiving numerous myocardial biopsies to check for rejection. One of our forensic pathologists is an expert in cardiovascular pathology and has read many biopsies over the years for other hospitals. His protocol for cutting these biopsies is very labor intensive (10 slides with 8 serial sections on each slide, some stained with H&E others kept unstained and 2 for C4d immunos).  I'm curious to know what other labs are doing for these biopsies in hopes of getting this protocol changes.

Thanks for your help

Jeff Miller
Spectrum Health
Grand Rapids. MI
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