[Histonet] (no subject)

[Jeff and Wanda Gray]

I would strongly suggest that you include cut resistant gloves as well.
These are usually plastic polymer of one sort or another. At my institution
we put on a pair of nitrile gloves, then the cut resistant ones, then
another pair of nitrile on size larger than we usually wear. Yes, it's a lot
to have on your, but yes, the extra safety is worth it. Many vendors sell
these, they are not hard to find at all.

Thanks,
Wanda S. Gray




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Message: 11
Date: Wed, 19 Jan 2011 10:28:36 -0600
From: <sgoebel <@t> mirnarx.com>
Subject: [Histonet] RE: Caspase
To: <LSetlak <@t> childrensmemorial.org>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<D957F2A7D21959488C492A2680F9920A1C5327 <@t> svrexch.asuragen.us>
Content-Type: text/plain;	charset="us-ascii"

I cc'd (is that a word?) the histonet for you.  If you ever want to pose
a question to everyone email: histonet <@t> lists.utsouthwestern.edu.

PPE: Of course everyone has different levels of PPE to be worn, but I
think the standard rule is...
Gloves
Lab Coat
Mask (especially if cutting frozen lung tissue, it could have TB)
Eye Wear
You have to remember you need all of this because once the tissue hits
room temperature it is basically fresh unfixed tissue again.
I have cut frozen sections with no mask before and it was all ok, but I
always wore one with lung frozens.
Good Luck, hope this helped

Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912




-----Original Message-----
From: Setlak, Lisa [mailto:LSetlak <@t> childrensmemorial.org] 
Sent: Wednesday, January 19, 2011 10:08 AM
To: Sarah Goebel
Subject: RE: Caspase

Hi Sarah,
I don't have an answer to your question but was wondering if you could
post one for me- I've tried multiple times and it doesn't seem to go
through. Would you be able to tell me how to pose a question to the
whole group or could you possibly post one "What type of PPE is normally
worn when performing frozen sections"? Thanks in advance for your help.
Lisa Van Valkenberg
Histology Mgr. 
Children's Memorial Hospital
Chg,. IL