[Histonet] H&E Stain

[John Kiernan]

Sounds as if the pH of your haemalum is too high. Try adding a little HCl to bring it down to slightly above 2. Check a few slides without eosin counterstaining. Nuclei should be blue with very little else stained.
 
John Kiernan
Anatomy & Cell Biology
University of Western Ontario
London, Canada
= = =
----- Original Message -----
From: "Scott, Allison D" <Allison_Scott <@t> hchd.tmc.edu>
Date: Wednesday, January 19, 2011 13:01
Subject: [Histonet] H&E Stain
To: histonet <@t> lists.utsouthwestern.edu

> Hello to all in histoland and Happy New Year.  We are 
> having issues with
> our H&E stain.  The nuclei are staining very blue to purple 
> and the
> mucin is staining blue to purple-blue.  It is difficult to 
> see the
> nuclear detail.  The mucin is obscuring things.  We 
> have not changed our
> process for staining or processing.  The funny thing is 
> that it is only
> in the Biopsy cases, and it is every few slides.  The 
> surgical  cases
> are all right.  We checked the alcohol and xylene for 
> water, and there
> is not any.  My tech changed out the stain and we are 
> staining a new
> batch of slides.  If anyone has any idea what is wrong, any 
> help would
> be greatly appreciated.  I have gone over our processes and 
> nothing has
> changed.  The reagents are the same, the staining times are 
> the same,
> and the processing times are the same.  We are using the 
> Shandon Gemini
> stainer and VIP processor.
> 
> Allison Scott HT(ASCP)
> Histology Supervisor
> LBJ Hospital
> Houston, Texas
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