[Histonet] alternative to Bouin's in Schiff-methylene blue nucleic acid

Carla M Conway cmconway <@t> usgs.gov
Wed Jan 19 14:15:56 CST 2011


A colleague is trying to determine the nucleic acid type of a rickettsial 
phage. Acridine orange with RNAse/DNAse treatment was not successful. They 
want to try a Schiff-methylene blue technique which requires hydrolyzing 
in Bouin's fluid (1 h at 60 C). Could anyone suggest another protocol 
which does not use picric acid (or suggest an alternative to Bouin's )? 

Also, any advice for nucleic acid differential staining would be 

Thanks very much, 

Carla Conway
Histology Technician
Western Fisheries Research Center, USGS
6505 N.E. 65th Street.
Seattle, WA 98115-5016 USA
Phone: 206-526-6282 ext. 242
Fax: 206-526-6654
E-mail: cmconway <@t> usgs.gov

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