[Histonet] H&E Stain

[Rathborne, Toni]

We have been having the same problem recently. We have tried extending the washes after the hematoxylin, agitation and adding an additional wash. Nothing has helped. It is not every slide as you say, but random ones. We are using Gill 3 and eosin from Stat Lab. We have changed the stainer and processor a number of times since this began.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Mike
Pence
Sent: Wednesday, January 19, 2011 1:10 PM
To: Scott, Allison D; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] H&E Stain


More often than I would like to admit when I have seen this type of
problem it has been that there is a solution out of place on the
processor or the stainer. I would start there.
Mike

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Scott,
Allison D
Sent: Wednesday, January 19, 2011 11:59 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] H&E Stain


Hello to all in histoland and Happy New Year.  We are having issues with
our H&E stain.  The nuclei are staining very blue to purple and the
mucin is staining blue to purple-blue.  It is difficult to see the
nuclear detail.  The mucin is obscuring things.  We have not changed our
process for staining or processing.  The funny thing is that it is only
in the Biopsy cases, and it is every few slides.  The surgical  cases
are all right.  We checked the alcohol and xylene for water, and there
is not any.  My tech changed out the stain and we are staining a new
batch of slides.  If anyone has any idea what is wrong, any help would
be greatly appreciated.  I have gone over our processes and nothing has
changed.  The reagents are the same, the staining times are the same,
and the processing times are the same.  We are using the Shandon Gemini
stainer and VIP processor.

Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas
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