[Histonet] Whole mount histology

[Rene J Buesa]

Dorothy:
First of all you have to be sure that the micrtotome you are going to use has enough cutting path to cover the embedded specimen.
You will also need the so called "mega cassettes" and "mega molds", unless you want to make them yourself out of plastic containers (for the cassettes) and of heavy aluminum foil (for the molds) as I first did in 1990 when I started the procedure at my laboratory. Instead of the molds you could use Leukhard's rectangles.
Finally you have to develop a fixation and processing protocol suitable for your specimens.
René J.

--- On Mon, 1/17/11, Webb, Dorothy L <Dorothy.L.Webb <@t> HealthPartners.Com> wrote:


From: Webb, Dorothy L <Dorothy.L.Webb <@t> HealthPartners.Com>
Subject: [Histonet] Whole mount histology
To: "'histonet <@t> lists.utsouthwestern.edu'" <histonet <@t> lists.utsouthwestern.edu>
Date: Monday, January 17, 2011, 10:51 AM


I am totally unfamiliar regarding whole mount histology as it pertains to prostate histology.  Can anyone in histoland assist me in finding information out regarding more specifics, especially in lieu of what costs would be incurred to implement this in a routine hospital histology department.

Appreciate any and all guidance!!

Dorothy Webb, HT
Regions Histology Technical Specialist  651-254-2962



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