[Histonet] RE: decalcifying bone marrows after processing

Garrison, Becky becky.garrison <@t> jax.ufl.edu
Fri Jan 14 16:41:03 CST 2011

We process bone marrow biopsies the same day received.   We use a combination decal/ fixative solution (Cal Rite decal) with good results.   We keep in this solution a minimum of 3 hours before moving to 
tissue processor where first 2 solutions are a total of 3 hours in 
formalin.    Although most bone marrow biopsies are received by 2:00 - 3:00pm; we've had some received as late as 4:00pm with good results.
Our clinicians place the bone marrow core in pre-filled formalin at the
point of collection, also.

The Cal Rite is a combination of formaldehyde, formic acid and methanol.  

Becky Garrison
Pathology Supervisor
Shands Jacksonville
Jacksonville, FL 32209
904-244-6237, phone
904-244-4290, fax
904-393-3194, pager
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Powers, Kerry
Sent: Friday, January 14, 2011 4:32 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] decalcifying bone marrows after processing

I was wondering if anyone has any experience with, or is it even possible to, decalcify bone marrows after they are processed.  Our pathologist would like to be able to process bone marrows the same day we receive them, but most of the time there just isn't enough time to allow for proper fixation and then proper decalcification.  She asked if we could process them and then decalcify and I have yet to find an answer to this question.  Please help!!
Thank you,
Kerry Powers
Comanche Country Memorial Hospital
Department of Pathology
3401 W Gore, Lawton OK 73505
(580) 355-8699 ext. 3359
Fax: (580) 585-5462
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