[Histonet] Thyroid Smears

Sharon.Davis-Devine Sharon.Davis-Devine <@t> carle.com
Wed Jan 12 11:02:03 CST 2011

Try using plus slides and make the smears very thin.  Then it won't matter what kind of fixative you are using everything will stick.

Sharon Davis-Devine, CT (ASCP)
Cytology-Histology  Supervisor
Carle Foundation Hospital
Laboratory and Pathology Services
611 West Park Street
Urbana, Illinois 61801
sharon.davis-devine <@t> carle.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of IRENA SREBOTNIK KIRBIS
Sent: Wednesday, January 12, 2011 10:55 AM
To: fawn.bomar <@t> halifaxregional.com; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Thyroid Smears

I guess that you're staining these fix slides by Papanicolaou?, perhaps the smears are too thick?, 
we process daily different kind of smears and always prepare one fix and one air dry smear for Pap and Giemsa staining and occasionaly we have an issue only very muccous samples which can coming off but never bloody smears except if they are very thick. 
one simples way to get rid of blood from cell suspension is a filtration through nylon mesh with 20 microns pores (published in Cytopathology - Heamorrhagic cytology samples- how to get best out of it)
> From: Fawn.Bomar <@t> HalifaxRegional.com
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Wed, 12 Jan 2011 09:33:06 -0500
> Subject: [Histonet] Thyroid Smears
> Hello Everyone!
> Happy New Years to all! I have a question regarding the preparation of thyroid smears. As of right now, we go up to the room and collect the thyroid sample. The Pathologist makes the smears in the room and immediately puts them into 95% Isopropanol to fix. We then complete the stain later on in the day. The problem that we are encountering is that all of the blood and cells are coming off of the slides before we make it through the entire stain. Does anyone have any suggestions or are willing to share the procedure that they use? We had a couple of suggestions that we recommended to the Doctor but they were dismissed. I don't want to tell what are suggestions were so that the doctor cannot accuse us of influencing every one else's opinions.
> Thank you in advance,
> Fawn
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