[Histonet] CD4 antibody for frozen mouse tissue

[Debrosse-Serra, Beatrice]

Hi Robin, 

We are using a biotinylated Anti-mouse CD4 from R&D Systems with great
success on frozen mouse tissue for IHC. The catalog number is BAM554. We
haven't used it in IF, but I would imagine it should work just as well
for multiplexing. Let me know if you need any more info.

Bea

Beatrice DeBrosse-Serra HT(ASCP)QIHC
Investigative Pathology Scientist 
Pfizer Global Research & Development
CB4, 2150
10646 Science Center Drive
San Diego, CA 92121
Phone# 858-622-5986
Fax# 858-678-8290
 
 
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Robin
Dean
Sent: Tuesday, January 11, 2011 3:30 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CD4 antibody for frozen mouse tissue

Can anyone recommend a good CD4 antibody for use in localizing CD4 in
frozen
mouse tissue?  We are being asked to do dual immunofluorescent (IHC)
labeling with the CD4 and another antibody in frozen mouse tissue.  I
know
CD4 localization in mouse tissue is difficult and would appreciate any
help
or guidance anyone might have to offer.

 

Thank you,

 

Robin 

 

Robin R. Dean, Ph.D.

Senior Scientist & Study Director

Comparative Biosciences, Inc.

786 Lucerne Dr.

Sunnyvale, CA

(408) 738-8060

robin_dean <@t> compbio.com

 

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