[Histonet] Chatter or waviness of sections under the 'scope

[kgrobert <@t> rci.rutgers.edu]

To all,

I am having a problem with either chatter or waviness of my sections when
I look at them under the microscope.  I've had chatter before, but in
these sections, the chattered areas of tissue are literally standing up,
giving the section a 3D look.  The waviness is the same thing, except the
tissue is in one piece-a 3D appearance under the microscope, no matter
what tissue I have cut. (It kind of looks to me like the way bacon curls
up in the pan when it's cooked-the fat in the bacon curls up and the meat
mostly remains flat.) So far I have seen it in mouse kidney, liver and
trachea.

Have my Ultrastick slides lost their charge, is it static electricity, bad
infiltration, or something else?  If you need to see a picture,let me know
& I'll go take one & post it.  Thank you so much!

Kathleen


Principal Lab Technician
Neurotoxicology Labs
Molecular Pathology Facility Core
Dept of Pharmacology & Toxicology
Rutgers, the State University of NJ
41 B Gordon Road
Piscataway, NJ 08854
(732) 445-6914