[Histonet] RE: Feulgen Stain for DNA

Della Speranza, Vinnie dellav <@t> musc.edu
Mon Jan 10 10:23:56 CST 2011

Feulgen demonstrates the deoxyribose which is in the backbone of each helical strand. It shouldn't matter if single or double stranded.
Hydrolysis is the key step and can be overdone. Textbooks list using hot 1N HCl
Many years ago Jules Elias and I co-authored a paper where we demonstrated that the use of 5N HCl at room temperature provided more consistent results 

Vinnie Della Speranza
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Liz Chlipala
Sent: Friday, January 07, 2011 4:00 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Feulgen Stain for DNA

Hello Everyone


I have tried to find this answer on the web and in references but I need
some help.  With respects to the Feulgen stain, how sensitive is it? Can
it detect smaller fragments of DNA? Does it have to be double stranded
or will single stranded stain as well?  Thanks in advance.




Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC


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