[Histonet] RE: Feulgen Stain for DNA

[Della Speranza, Vinnie]

Feulgen demonstrates the deoxyribose which is in the backbone of each helical strand. It shouldn't matter if single or double stranded.
Hydrolysis is the key step and can be overdone. Textbooks list using hot 1N HCl
Many years ago Jules Elias and I co-authored a paper where we demonstrated that the use of 5N HCl at room temperature provided more consistent results 

Vinnie Della Speranza
Manager for Anatomic Pathology Services
165 Ashley Avenue Suite 309
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974
  
           
              Learn more about Histotechnology Professionals Day at 
                                            www.nsh.org
 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Liz Chlipala
Sent: Friday, January 07, 2011 4:00 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Feulgen Stain for DNA

Hello Everyone

 

I have tried to find this answer on the web and in references but I need
some help.  With respects to the Feulgen stain, how sensitive is it? Can
it detect smaller fragments of DNA? Does it have to be double stranded
or will single stranded stain as well?  Thanks in advance.

 

Liz

 

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC

Manager

Premier Laboratory, LLC

PO Box 18592

Boulder, Colorado 80308

office (303) 682-3949 

fax (303) 682-9060

www.premierlab.com

 

 

Ship to Address:

1567 Skyway Drive, Unit E

Longmont, Colorado 80504

 

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet