[Histonet] Charged slides

[Marvin Hanna]

Hi Helen,

Plus is the branding for silanized slides, so they are the same. As  
far as magical, I'm not sure, but the silanization procedure  
covalently bonds the amino groups in APES (3- 
Aminopropyltriethoxysilane, available from Sigma) to the silicate  
ions on the surface of the glass, creating a positive charge on the  
slide.

Polylysine coating also puts a positive charge on the slide, but it  
is just a coating, unlike the chemically reacted covalent bond of the  
silanization procedure.

A positive charge on the surface of the slide allows for strong  
bonding with negatively charged formalin fixed tissue.

John Kiernan wrote a paper many years back on adhesives for slides  
that shows the procedure and chemical reaction in silanizing slides.  
It is available here.
http://publish.uwo.ca/~jkiernan/adhesivs.htm

Kind Regards,

Marvin Hanna


On Jan 6, 2011, at 5:43 PM, Helen Fedor wrote:

> Hi, A question has come up regarding the different methods used to  
> put a charge on the slide. We recently ordered some plus slides and  
> the boxes they are packed in say silanized slides, but they say   
> "plus" on the slide . We don't want to use these for our clients if  
> they are not going to be getting the same results as the former  
> "plus" slides that we were using. I was under the delusion that  
> Plus slides somehow are magically charged without any coating  
> process taking place.
>
> So does anyone know exactly how a "plus" slide gets its charge? Do  
> they all get dipped in APES, or Polylysine?
>
> Thanks for your help.
>
> --
> Helen
>
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