[Histonet] Re: Histonet Digest, Vol 86, Issue 2

[MKing]

It may however if you're doing immunofluorescence, as MB has been used 
to quench autofluorescence (Seeing the Wood through the Trees: A Review 
of Techniques for Distinguishing Green Fluorescent Protein from 
Endogenous Autofluorescence; Nicholas Billinton and Andrew W. Knight, 
Analytical Biochemistry 291, 175–197 (2001).  It isn't likely to alter 
antigen-antibody binding if that's what you meant.

Mike King
UF Pharmacology & Therapeutics
> 
> Message: 1
> Date: Sat, 1 Jan 2011 15:37:32 -0700
> From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
> Subject: RE: [Histonet] methylene blue stained tissue
> To: "'Quinn, Tim L.'" <quinntl <@t> umkc.edu>,
> 	<histonet <@t> lists.utsouthwestern.edu>
> Cc: "'Herndon, Betty L.'" <HerndonB <@t> umkc.edu>
> Message-ID: <47914CBC2E9B4DEB80235F70DF65B7DA <@t> prueggihctechlt>
> Content-Type: text/plain;	charset="us-ascii"
> 
> Should not be a problem if you are using hrp/dab brown or ap/red detection.
> 
> Patsy
> 
> Patsy Ruegg, HT(ASCP)QIHC
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Quinn, Tim
> L.
> Sent: Thursday, December 30, 2010 12:30 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Cc: Herndon, Betty L.
> Subject: [Histonet] methylene blue stained tissue
> 
> Hello Histies,
> 
> I received rodent dermis that has been stained with methylene blue.
> 
> I would like to perform immunolabeling experiments on this tissue.
> 
> Should I expect any interference from the methylene blue stain?
> 
> Tim Quinn
>