[Histonet] Sirius red stain - picric acid substitute - water -really?

[Marcum, Pamela A]

Since you can buy a saturated Picric acid solution and I have for years why are you worried?  It is stable in a saturated liquid and you can purchase fairly small amounts at a time.  I only worry if find some in a powder form turning a nasty colour.  It has been about twenty five years since I had that happen and immediately went to pre-mixed solutions.

Pam Marcum
UAMS

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret 
Sent: Wednesday, February 23, 2011 8:04 AM
To: Mia Woodruff; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Sirius red stain - picric acid substitute - water -really?

I would do a trial run on "extra" slides or non-valuable tissue? 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mia Woodruff
Sent: Tuesday, February 22, 2011 10:33 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Sirius red stain - picric acid substitute - water -really?

Hello all,

I have been undertaking sirius red staining using picric acid, I was under the
impression (from papers) that picric acid is an important component of the
procedure and read that it prevents non-specific binding of the dye to things
other than collagen. However,  I have recently found a paper which suggests I
can simply use water instead of picric acid, seems quite a long shot but I
wondered if anyone has experience with the water technique- given that picric
acid is pretty dangerous I would be keen to move away from using it but only if
scientifically sound to do so. I don't want to jeopardize my results but if this
method works then it's a far safer and cheaper safer approach. Any advice?

Paper: A specific quantitative assay for collagen synthesis by cells seeded in
collagen-based biomaterials using sirius red F3B precipitation
LEE D.A.; ASSOKU E.; DOYLE V. Journal of Materials Science: Materials in
Medicine<http://www.ingentaconnect.com/content/klu/jmsm>, Volume 9, Number 1,
1998 , pp. 47-51(5)


Many thanks
Mia





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