SPAM-LOW: [Histonet] TRAP staining kit

Patsy Ruegg pruegg <@t>
Mon Feb 21 11:46:00 CST 2011

Kim if you are asking if TRAP enzyme histochemistry works on formalin fixed
paraffin embedded decalcified bone in my experience it does not.  It works
on smears and cytology slides or GMA embedded bone without decal, it may
work on ETA decaled bone but I do not think it works on ffpe slides.  There
is an antibody to TRAP now you could use on ffpe sections by IHC.  What are
you trying to use it for, osteoclasts???


Patsy Ruegg, HT(ASCP)QIHC
12635 Montview Blvd. Ste.215
Aurora, CO 80045
fax 720-859-4110

-----Original Message-----
From: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] On Behalf Of Kim Merriam
Sent: Monday, February 21, 2011 7:39 AM
To: Histonet
Subject: SPAM-LOW: [Histonet] TRAP staining kit

Hi All,
Does anyone know if there is a TRAP staining kit available for histology
who sells it)?  I have found a bunch of them on google, but they are all for

cell culture.
 Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 

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