[Histonet] RE: Histonet Digest, Vol 87, Issue 33
Goodwin, Diana
dgoodwin <@t> rwjuhh.edu
Fri Feb 18 12:45:36 CST 2011
We are also fans of the Leica ASP 300 processor.
Diana G. Goodwin, BS, HT(ASCP)QIHC
Department of Pathology
Robert Wood Johnson University Hospital at Hamilton
Hamilton, NJ 08610
609-631-6996
dgoodwin <@t> rwjuhh.edu
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Sent: Friday, February 18, 2011 1:05 PM
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Subject: Histonet Digest, Vol 87, Issue 33
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Today's Topics:
1. Tissue Processors (Joe Nocito)
2. RE: Tissue Processors (Langenberg, Stacey)
3. IHC pretreatment with NaOH/H2O2 (Neil M. Fournier)
4. Eosinophilic new bone formation (Keller, Pat)
5. Re: Tissue Processors (Rene J Buesa)
6. RE: Tissue Processors (Nails, Felton)
7. RE: Tissue Processors (Houston, Ronald)
8. Re: Eosinophilic new bone formation (Jack Ratliff)
9. Histotechnologist with a FL HTL license for Fort Myers, FL
(Brian- Prometheus)
10. RE: Tissue Processors (sgoebel <@t> mirnarx.com)
11. Microwave Usage, Monitoring procedure (Scott, Allison D)
----------------------------------------------------------------------
Message: 1
Date: Thu, 17 Feb 2011 19:55:53 -0600
From: "Joe Nocito" <jnocito <@t> satx.rr.com>
Subject: [Histonet] Tissue Processors
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <48ABF837D7084DCCB45CADF5F2B3F20A <@t> JoePC>
Content-Type: text/plain; charset="iso-8859-1"
Greetings all,
if you had to purchase new tissue processors, which one would you choose? Microwave technology is out of the question. Are Sakura's still a good buy? We've tried the Leica Peloris and the Shandon Pathcenters. Thanks for your help
Joe
------------------------------
Message: 2
Date: Thu, 17 Feb 2011 19:33:13 -0700
From: "Langenberg, Stacey" <STACEY.LANGENBERG <@t> UCDENVER.EDU>
Subject: RE: [Histonet] Tissue Processors
To: Joe Nocito <jnocito <@t> satx.rr.com>, Histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1F70FCBB6D4EC549B2ADF69B9F9EAC034E6B03F404 <@t> STEAMBOAT.ucdenver.pvt>
Content-Type: text/plain; charset="us-ascii"
Joe,
We are really impressed with the Leica ASP 300. We have a second one on the way.
Stacey
"People are not an interruption of our business. People are our business."
Stacey Langenberg HT (ASCP) QIHC
Laboratory Manager
Histology/IF
CU Dermatopathology Consultants
1999 N. Fitzsimons Pkwy Suite 120
Aurora, CO 80045
Lab-720-859-3559 Fax- 303-344-0789 Office- 303-577-2303 Cell-970-405-7742
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joe Nocito [jnocito <@t> satx.rr.com]
Sent: Thursday, February 17, 2011 6:55 PM
To: Histonet
Subject: [Histonet] Tissue Processors
Greetings all,
if you had to purchase new tissue processors, which one would you choose? Microwave technology is out of the question. Are Sakura's still a good buy? We've tried the Leica Peloris and the Shandon Pathcenters. Thanks for your help
Joe
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Histonet <@t> lists.utsouthwestern.edu
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------------------------------
Message: 3
Date: Fri, 18 Feb 2011 00:53:30 -0500
From: "Neil M. Fournier" <neil.fournier <@t> yale.edu>
Subject: [Histonet] IHC pretreatment with NaOH/H2O2
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20110218005330.8h3p3wjcu84gc484 <@t> www.mail.yale.edu>
Content-Type: text/plain; charset=ISO-8859-1
I recently came across a protocol that was conducting IHC staining for pSTAT3 on
free-floating fixed rat brain sections. The protocol stated that the tissue was
placed in 1% NaOH and 1% H2O2 in H2O for 20 min, 0.3% glycine for 10 min, and
0.03% sodium dodecyl sulfate for 10 min.
Could someone please explain to me the rationale for using NaOH and H2O2
pretreatment, as well as the additional steps in this procedure? My rationale
may be incorrect but I would be concerned that the combination of NaOH and H2O2
might produce a too much O2 bubbling that could harm the tissue. I believe
glycine is typically used for reduce autofluoresence since it binds to free
aldehydes but this protocol was utilizing a cobolt/nickel enhanced-DAB reaction
and I am unaware of any empirical study that has shown that glycine pretreatment
improves peroxidase immunostaining. Finally, SDS seems like a fairly harsh
detergent (although the remaining protocol utilizes Triton X-100 in blocking
and antibody incubation steps), has anyone used this? and what would be the
benefit of this over other detergents.
Thanks in advance
Neil
------------------------------
Message: 4
Date: Fri, 18 Feb 2011 06:45:58 -0600
From: "Keller, Pat" <KellerP <@t> ent.wustl.edu>
Subject: [Histonet] Eosinophilic new bone formation
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<F6712DAB9953564CB2C8C5813BC980836B2D57 <@t> EX03.wusm-pcf.wustl.edu>
Content-Type: text/plain; charset="us-ascii"
We have always noticed, at least in the middle/inner ear, that newly
deposited bone stains darker than mature bone, both with H&E and
toluidine blue. Is this increased eosinophilic quality due to a lack of
mineralization and therefore higher density of osteoid components in the
new bone or some other difference in composition of the osteoid? The
contrast is quite striking when we observe bone remodeling due to middle
ear infections, so I wanted to be able to offer an accurate explanation
of why that is...
Patricia Keller
Sr. Research Tech/Core Histologist
Washington University School of Medicine
Department of Otolaryngology
4566 Scott Ave
Campus Box 8115
St. Louis, Mo 63110
314-747-7166
This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail.
------------------------------
Message: 5
Date: Fri, 18 Feb 2011 05:33:27 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Tissue Processors
To: Histonet <histonet <@t> lists.utsouthwestern.edu>, Joe Nocito
<jnocito <@t> satx.rr.com>
Message-ID: <366928.38959.qm <@t> web65711.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Sahura is still the best buy (for me).
Ren? J.
--- On Thu, 2/17/11, Joe Nocito <jnocito <@t> satx.rr.com> wrote:
From: Joe Nocito <jnocito <@t> satx.rr.com>
Subject: [Histonet] Tissue Processors
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Date: Thursday, February 17, 2011, 8:55 PM
Greetings all,
if you had to purchase new tissue processors, which one would you choose? Microwave technology is out of the question. Are Sakura's still a good buy? We've tried the Leica Peloris and the Shandon Pathcenters. Thanks for your help
Joe
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Fri, 18 Feb 2011 07:59:44 -0600
From: "Nails, Felton" <flnails <@t> texaschildrens.org>
Subject: RE: [Histonet] Tissue Processors
To: "'Joe Nocito'" <jnocito <@t> satx.rr.com>, "Histonet"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C1FE5960057C084CA389CE977790629091F8F08C <@t> TCDMSG01.ad.TexasChildrensHospital.org>
Content-Type: text/plain; charset=us-ascii
Joe, I just purchased a VIP6 because of my experience with this brand both while in the military and civilian life.
Have never had a problem with them.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joe Nocito
Sent: Thursday, February 17, 2011 7:56 PM
To: Histonet
Subject: [Histonet] Tissue Processors
Greetings all,
if you had to purchase new tissue processors, which one would you choose? Microwave technology is out of the question. Are Sakura's still a good buy? We've tried the Leica Peloris and the Shandon Pathcenters. Thanks for your help
Joe
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Message: 7
Date: Fri, 18 Feb 2011 09:16:37 -0500
From: "Houston, Ronald" <Ronald.Houston <@t> nationwidechildrens.org>
Subject: RE: [Histonet] Tissue Processors
To: 'Rene J Buesa' <rjbuesa <@t> yahoo.com>, Histonet
<histonet <@t> lists.utsouthwestern.edu>, Joe Nocito <jnocito <@t> satx.rr.com>
Message-ID:
<E02E1309B208F94C83B968E45781001A234C53BDF8 <@t> NCHEXMBX01.columbuschildrens.net>
Content-Type: text/plain; charset="iso-8859-1"
I would probably have gone along with you on this Rene but since we demo'ed, and subsequently purchased, the Peloris my opinion has changed
Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, February 18, 2011 8:33 AM
To: Histonet; Joe Nocito
Subject: Re: [Histonet] Tissue Processors
Sahura is still the best buy (for me).
Ren? J.
--- On Thu, 2/17/11, Joe Nocito <jnocito <@t> satx.rr.com> wrote:
From: Joe Nocito <jnocito <@t> satx.rr.com>
Subject: [Histonet] Tissue Processors
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Date: Thursday, February 17, 2011, 8:55 PM
Greetings all,
if you had to purchase new tissue processors, which one would you choose? Microwave technology is out of the question. Are Sakura's still a good buy? We've tried the Leica Peloris and the Shandon Pathcenters. Thanks for your help
Joe
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Message: 8
Date: Fri, 18 Feb 2011 08:37:36 -0600
From: Jack Ratliff <ratliffjack <@t> hotmail.com>
Subject: Re: [Histonet] Eosinophilic new bone formation
To: "Keller, Pat" <KellerP <@t> ent.wustl.edu>
Cc: "<histonet <@t> lists.utsouthwestern.edu>"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BLU0-SMTP1767D494C3052E29F41F175AED40 <@t> phx.gbl>
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In decalcified sections of bone, yes the osteoid or dense unmineralized collagen matrix (mostly type I) will stain darker than mature native demineralized bone. Even though the mature bone has been demineralized, it is still more densely compact as compared to the newly formed bone that has not begun mineralization and . In resin embedded undemineralized sections of bone, the contrast is exactly opposite when staining with Von Kossa and counterstaining with MacNeal's tetrachrome and similar to decalcified sections in a Goldner's trichrome where the acid fuchsin stains osteoid darker than the light green does the mineralized bone. So the answer is yes, tissue density is what plays the major role in contrast staining and stain intensity.
Jack
On Feb 18, 2011, at 6:45 AM, "Keller, Pat" <KellerP <@t> ent.wustl.edu> wrote:
> We have always noticed, at least in the middle/inner ear, that newly
> deposited bone stains darker than mature bone, both with H&E and
> toluidine blue. Is this increased eosinophilic quality due to a lack of
> mineralization and therefore higher density of osteoid components in the
> new bone or some other difference in composition of the osteoid? The
> contrast is quite striking when we observe bone remodeling due to middle
> ear infections, so I wanted to be able to offer an accurate explanation
> of why that is...
>
>
>
>
>
> Patricia Keller
> Sr. Research Tech/Core Histologist
> Washington University School of Medicine
> Department of Otolaryngology
> 4566 Scott Ave
> Campus Box 8115
> St. Louis, Mo 63110
> 314-747-7166
>
>
>
> This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 9
Date: Fri, 18 Feb 2011 10:33:29 -0500
From: "Brian- Prometheus" <brian <@t> prometheushealthcare.com>
Subject: [Histonet] Histotechnologist with a FL HTL license for Fort
Myers, FL
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <008101cbcf81$344c5660$9ce50320$@com>
Content-Type: text/plain; charset="us-ascii"
Looking for a Histotechnologist with a FL HTL license for Fort Myers, FL.
This will be a late second or third shift. Experience with IHC and Ventanna
is preferred.
Please contact me today for immediate consideration.
Brian Feldman
Principal
Prometheus Healthcare
Office 301-693-9057
Fax 301-368-2478
brian <@t> prometheushealthcare.com <mailto:brian <@t> prometheushealthcare.com>
www.prometheushealthcare.com <http://www.prometheushealthcare.com/>
*** Stay up to date on the newest positions and healthcare trends nationwide
on Twitter!***
http://twitter.com/PrometheusBlog
------------------------------
Message: 10
Date: Fri, 18 Feb 2011 10:00:18 -0600
From: <sgoebel <@t> mirnarx.com>
Subject: RE: [Histonet] Tissue Processors
To: <jnocito <@t> satx.rr.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<D957F2A7D21959488C492A2680F9920A1C5982 <@t> svrexch.asuragen.us>
Content-Type: text/plain; charset="US-ASCII"
Pathcenters in my opinion don't last as long?
Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joe
Nocito
Sent: Thursday, February 17, 2011 7:56 PM
To: Histonet
Subject: [Histonet] Tissue Processors
Greetings all,
if you had to purchase new tissue processors, which one would you
choose? Microwave technology is out of the question. Are Sakura's still
a good buy? We've tried the Leica Peloris and the Shandon Pathcenters.
Thanks for your help
Joe
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 11
Date: Fri, 18 Feb 2011 10:47:56 -0600
From: "Scott, Allison D" <Allison_Scott <@t> hchd.tmc.edu>
Subject: [Histonet] Microwave Usage, Monitoring procedure
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1872B4A455B7974391609AD8034C79FC026DFD69 <@t> LBEXCH01.hchd.local>
Content-Type: text/plain; charset="us-ascii"
Hello to all in histoland. I am in need of a procedure for the usgae,
monitoring and container venting of the mcrowave. I have a procedure
that I wrote several years ago, but my boss wants it updated. Any help
will be appreciated.
Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas
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