[Histonet] RE: neutrophil Ab for rat FFPE

[PALMER Jason (SVHM)]

Amy,

We have used Abcam rabbit anti human myeloperoxidase (Ab9535) with very good results on FFPE rat tissue.  No retrieval required, primary at 1:50, biotinylated secondary and ABC elite with DAB for detection.  Hope that helps.

Jason


Jason Palmer
Histology Laboratory Coordinator
O'Brien Institute
42 Fitzroy St, Fitzroy Victoria 3065
Australia
tel +61 3 9288 4045
fax +61 3 9416 0926
email: jason.palmer <@t> svhm.org.au

Message: 7
Date: Wed, 16 Feb 2011 12:06:41 -0800 (PST)
From: Amy Lee <amylee779 <@t> yahoo.com>
Subject: [Histonet] neutrophil antibody on rat FFP section
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <544851.36245.qm <@t> web38005.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hello,
I was asked to perform neutrophil IHC on rat FFP sections. I searched around and found most are for mouse tissue. There is one from Serotec mouse anti rat but it does not work on paraffin section.
?
Could anybody recommend a good antibody?
?
Thanks in advance,
?
Amy

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