[Histonet] Section position on slides

[Victoria Baker]

Automated coverslipping instruments are defined by the manufacturer, but
modifications on some systems can be done.  Most clinical labs who utilize
automation use 24X50mm - as this coverslip will cover most of the slide.

Tissue placement is pretty much determined by laboratory needs.  Sheehan &
Hrapchak had a short segment about tissue placement, but it was in regards
to the tissue orientation for microscopic assessment requirements.  There is
nothing in granite about tissue placement that I know of.

Some tools that I worked with were using the Cytospin large rectangular
slides to show residents/students/collegues where the tissue placement
needed to be.  Once they were able to get the tissue section in there we
moved on.  While to most of us who work in Histo sort of take putting
sections on a slide for granted (yes we did work hard to get there)  getting
the mechanics of it takes patience, skill and practice.  Keep it simple and
build up from there.

Vikki


On Wed, Feb 16, 2011 at 12:27 PM, Helen Fedor <hfedor <@t> jhmi.edu> wrote:

> Since automation is becoming more and more a part of all Histology labs the
> demands of placement of the tissue on the slides varies for different
> instruments. Stainers, coverslippers and now with slide scanning as well. So
> I do not believe that there is a silver bullet answer.
>
> Helen L. Fedor
>
> Tissue Microarray Lab, Manager
> Prostate Spore Lab, Manager
> Johns Hopkins University
> 600 N. Wolfe St, | Marburg Room 406
> Baltimore, MD | 21287-7065
>
> 410.614.1660
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine
> (CDC/OID/NCEZID)
> Sent: Wednesday, February 16, 2011 12:21 PM
> To: Tanya Ewing-Finchem; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Section position on slides
>
> I always try to center the section equally from sides and top-bottom as
> possible. This means measure from the bottom of the frosted edge as the
> "top".  The Artisan special stains system has a clip that attaches around
> the slide to allow reagents to pool onto the sections and incubate.  If the
> section is too close to the sides then these areas do not stain adequately.
>  Sometimes if the tissue section itself is very large this is unavoidable.
>  With automated coverslippers you must also consider placement of tissue to
> allow for proper coverage.
>
> If I am cutting multiple unstained slides for subsequent testing I try to
> orient the tissue the same on each slide to facilitate the reading of these
> slides by the pathologist's
>
> Jeanine Bartlett
> Infectious Diseases Pathology Branch
> (404) 639-3590
> jeanine.bartlett <@t> cdc.hhs.gov
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tanya
> Ewing-Finchem
> Sent: Wednesday, February 16, 2011 12:09 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Section position on slides
>
>
> I am trying to put together a training document around microtomy and
> sectioning and am finding it hard to find information around the placement
> of the actual sections on the slides.  These are the objectives I am looking
> to answer.  Is this information found in any publications?
>
> 1)  Tissue / Section Placement:  Are there published guidelines /
> documentation on precisely where you should place tissue sections on a 25mm
> x 75mm glass slide?   Perhaps more importantly, where you should NOT place
> tissue (ie. "x" mm from the edge of the glass slide)?
>
> 2)  Diagnosable Slide Staining Area:  With automation becoming more widely
> used in IHC, are there published guidelines / documentation on the usable or
> diagnosable staining area on a 25mm x 75mm glass slide?  For instance, would
> you define that as the area under a traditional coverslip?  Would this be
> defined as the entire slide below the label?  Or is this some distance from
> all the edges of the slide?  With some automated systems, it is near
> impossible to get edge to edge staining.  Is this acceptable?
>
>
>
> Thanks for any ideas.
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