[Histonet] Re: mouse skeletal muscle frozen section prep

Robert Richmond rsrichmond <@t> gmail.com
Sat Feb 12 12:28:02 CST 2011

Before you freeze a muscle specimen, you coat it thinly with talc,
pick it up with tweezers, and bob it up and down about ten times in
the freezing liquid (not directly in liquid nitrogen), then mount it
in OCT or other freeze-mount medium.

Correctly done, this will largely avoid bubble artifact. It takes a
few times to get the knack of it - best to practice with some junk
muscle (from a human amputation specimen, or from a dead animal of
your choice) to get it right before you do it on an actual biopsy
specimen or on valuable research material.

Bob Richmond
Samurai Pathologist
Knoxville TN

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