[Histonet] FFPE tissue - sucrose fixing necessary?

[Abbey Mortimer]

Thanks for your responses :)

Abbey

________________________________________
From: Margaret Blount [mab70 <@t> medschl.cam.ac.uk]
Sent: Thursday, 10 February 2011 7:21 PM
To: Abbey Mortimer; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] FFPE tissue - sucrose fixing necessary?

This sounds like something you would do before freezing not processing
to paraffin.

Miss Margaret Blount
Histology Manager
Metabolic Research Laboratories
Level 4 Institute of Metabolic Science
Box 289, Addenbrooke's Hospital
Hills Road, Cambridge, CB2 0QQ

Tel 01223 769061/336079


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Abbey
Mortimer
Sent: 10 February 2011 02:22
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] FFPE tissue - sucrose fixing necessary?

Hello helfpul Histonetters!
I am curious about your formalin-fixed paraffin embedded (FFPE) tissue
protocols. If the tissue has been fixed with saline and paraformaldehyde
during perfusion, then further fixed in paraformaldehyde for 24 hours,
do you still utilise a sucrose wash before running it through the
automatic tissue processor? Or do you not need this step? I have heard
mixed responses about this and wonder if any of you have had experience
with this!

Thanks so much,
Abbey
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet