[Histonet] FFPE tissue - sucrose fixing necessary?

Abbey Mortimer A.Mortimer <@t> latrobe.edu.au
Thu Feb 10 03:41:37 CST 2011

Thanks for your responses :)


From: Margaret Blount [mab70 <@t> medschl.cam.ac.uk]
Sent: Thursday, 10 February 2011 7:21 PM
To: Abbey Mortimer; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] FFPE tissue - sucrose fixing necessary?

This sounds like something you would do before freezing not processing
to paraffin.

Miss Margaret Blount
Histology Manager
Metabolic Research Laboratories
Level 4 Institute of Metabolic Science
Box 289, Addenbrooke's Hospital
Hills Road, Cambridge, CB2 0QQ

Tel 01223 769061/336079

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Abbey
Sent: 10 February 2011 02:22
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] FFPE tissue - sucrose fixing necessary?

Hello helfpul Histonetters!
I am curious about your formalin-fixed paraffin embedded (FFPE) tissue
protocols. If the tissue has been fixed with saline and paraformaldehyde
during perfusion, then further fixed in paraformaldehyde for 24 hours,
do you still utilise a sucrose wash before running it through the
automatic tissue processor? Or do you not need this step? I have heard
mixed responses about this and wonder if any of you have had experience
with this!

Thanks so much,
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Histonet <@t> lists.utsouthwestern.edu

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