[Histonet] Re: Amylase Digestion for glycogen

[Nathan Jentsch]

We use amylase digestion for our daily PAS/D stains.  The procedure is not
that complicated.  We preheat the enzyme in a 45 degree C water bath for 10
minutes.  Then we let the slides digest with the solution still in the water
bath for 10 minutes.  Then we rinse in running tap water for 5 minutes and
proceed to the periodic acid.
Nathan Jentsch
BS HT(ASCP)