[Histonet] Where can "Fast Blue" (Diamidino compound 253/50) be obtained?

koellingr <@t> comcast.net koellingr <@t> comcast.net
Sat Feb 5 09:20:01 CST 2011

Hello Per, 

Have no particular interest in neuronal retrograde tracing and have certainly never used anything for that. Just general scientific curiosity and a few minutes time awaiting a home project.  Found Fast Blue (synonym: Diamidino compound 253/50) on Sigma website cat # F5756. Good luck. 


Ray Koelling 

PhenoPath Labs 

Seattle WA 

----- Original Message ----- 
From: "Per Magne Knutsen" <pknutsen <@t> physics.ucsd.edu> 
To: histonet <@t> lists.utsouthwestern.edu 
Sent: Friday, February 4, 2011 11:22:41 PM 
Subject: [Histonet] Where can "Fast Blue" (Diamidino compound 253/50) be        obtained? 

I've searched every catalog and website for "Fast Blue" (Diamidino compound 
253/50), widely used for neuronal retrograde tracing. One recent paper using 
this compound is: 

Porreroa et al "Mapping of fluorescent protein-expressing neurons and axon 
pathways in adult and developing Thy1-eYFP-H transgenic mice", Brain 
Research, 1345, 59-72 

where the authors as many before them cite "Dr. Illing GmbH & Co. KG, 
Groβ-Umstadt, Germany" as the origin. I have been unable to locate this 
particular vendor. I've written the authors and am waiting for an answer. 

In the meanwhile, can anyone on this list help? 


*Per M Knutsen 
*Department of Physics 
University of California, San Diego 
9500 Gilman Drive, La Jolla, CA 92093-0374 

T: +1 858 405 2868 
E: pknutsen <@t> ucsd.edu 
W: http://pmknutsen.blogspot.com 
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