[Histonet] Bond Refine Red for manual IHC

Houston, Ronald Ronald.Houston <@t> nationwidechildrens.org
Fri Feb 4 09:39:11 CST 2011

Yes, we've used both the AP Red and DAB Refine kits manually with comparable results to the automation

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sarka Lhotak
Sent: Friday, February 04, 2011 10:18 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Bond Refine Red for manual IHC

Hello all,

I wonder if anyone is using the Bond Polymer Refine Red Detection kit
(cat # DS390, Leica) for manual IHC. I've seen some beautiful staining,
the technical specialist at Leica, however, told me it is only for
Leica automatic stainers. It is an AP stain. 
Has anybody tried it?

Thanks a lot,

Sarka Lhotak
McMaster University
Hamilton, Ontario, Canada

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