[Histonet] Re: Histonet Digest, Vol 87, Issue 7

Fri Feb 4 08:14:39 CST 2011

Patty  view utube  if you can.

------Original Message------
From:	"histonet-request <@t> lists.utsouthwestern.edu" <histonet-request <@t> lists.utsouthwestern.edu>
Date:	Fri Feb 04, 2011 -- 04:58:02 AM
To:	histonet <@t> lists.utsouthwestern.edu
Subject:	Histonet Digest, Vol 87, Issue 7

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Today's Topics:

   1. Formaldehyde monitor (Bob Nienhuis)
   2. Consult accessioning question (Richard Cartun)
   3. Re: Consult accessioning question (Victor Tobias)
   4. RE: Consult accessioning question (Wanda.Smith <@t> HCAhealthcare.com)
   5. Re: Formaldehyde monitor (Kim.Donadio <@t> bhcpns.org)
   6. RE: Formaldehyde monitor (Wanda.Smith <@t> HCAhealthcare.com)
   7. RE: Consult accessioning question (Mike Pence)
   8. Re: Off Topic: researchers very funny (Merced M Leiker)
   9. RE: Consult accessioning question (Weems, Joyce)
  10. TTF-1/background staining (Paula Lucas)
  11. Microwave processing (Webb, Dorothy L)
  12. FW: [Histonet] Formaldehyde monitor (Vanessa Avalos)
  13. Re: Microwave processing (Rene J Buesa)
  14. Temporary histology position in San Diego Ca. (James Watson)
  15. RE: TTF-1/background staining (Debra Siena)
  16. RE: Her2 Fixation Requirement  (Rathborne, Toni)
  17. Karnovsky and Roots stain (Nicole Cosenza)
  18. RE: TTF-1/background staining (Anthony Reilly)
  19. Re: TTF-1/background staining (Mark Tarango)
  20. thionin staining (An Eerdekens)
  21. Cre protocol-fresh frozen section (James Dooley)
  22. RE: CLIA classes? (erika <@t> mapslab.net)

----------------------------------------------------------------------

Message: 1
Date: Thu, 3 Feb 2011 10:29:18 -0800
From: Bob Nienhuis <bob.nienhuis <@t> gmail.com>
Subject: [Histonet] Formaldehyde monitor
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<AANLkTimcGcnxpOpCQHop5WwxXPyvu1Mwo=UkaNJsqUvT <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Anyone know of a cheap formaldehyde gas monitor or concentration test
method?

I found a couple of monitors on Google, but they seem to run abour $1500+

Bob
Sepulveda VA Med Center
Los Angeles

------------------------------

Message: 2
Date: Thu, 03 Feb 2011 13:43:05 -0500
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
Subject: [Histonet] Consult accessioning question
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4D4AB0E8.7400.0077.1 <@t> harthosp.org>
Content-Type: text/plain; charset=US-ASCII

When accessioning a case for consultation, do you use the date on the paperwork from the referring hospital/pathologist as the new "Date-of-service"?  If so, what do you do when the paperwork gives a date (say Friday) and then the specimen is not sent out until the following Monday?  Thank you.

Richard 

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax

------------------------------

Message: 3
Date: Thu, 03 Feb 2011 10:59:53 -0800
From: Victor Tobias <victor <@t> pathology.washington.edu>
Subject: Re: [Histonet] Consult accessioning question
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4D4AFB29.8070601 <@t> pathology.washington.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

Richard,

We use the date of accessioning as the DOS and we wouldn't accession it 
until we have received the specimen/materials.

Victor

Victor Tobias HT(ASCP)
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
victor <@t> pathology.washington.edu
206-598-2792
206-598-7659 Fax
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On 2/3/2011 10:43 AM, Richard Cartun wrote:
> When accessioning a case for consultation, do you use the date on the paperwork from the referring hospital/pathologist as the new "Date-of-service"?  If so, what do you do when the paperwork gives a date (say Friday) and then the specimen is not sent out until the following Monday?  Thank you.
>
> Richard
>
> Richard W. Cartun, MS, PhD
> Director, Histology&  Immunopathology
> Director, Biospecimen Collection Programs
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT  06102
> (860) 545-1596 Office
> (860) 545-2204 Fax
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 4
Date: Thu, 3 Feb 2011 13:06:14 -0600
From: <Wanda.Smith <@t> HCAhealthcare.com>
Subject: RE: [Histonet] Consult accessioning question
To: <Rcartun <@t> harthosp.org>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<9E2D36CE2D7CBA4A94D9B22E8328A3BA139EBA807A <@t> NADCWPMSGCMS03.hca.corpad.net>

Content-Type: text/plain; charset="us-ascii"

In our LIS system we put in "specimen date" or DOS and we also have a "Received date".  My understanding, for insurance/billing purposes, you would need to include the original DOS, but you also need to be able to document when you actually received the specimen.
Just my 2 cents!!!
Wanda

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Richard Cartun
Sent: Thursday, February 03, 2011 1:43 PM
To: Histonet
Subject: [Histonet] Consult accessioning question

When accessioning a case for consultation, do you use the date on the paperwork from the referring hospital/pathologist as the new "Date-of-service"?  If so, what do you do when the paperwork gives a date (say Friday) and then the specimen is not sent out until the following Monday?  Thank you.

Richard 

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 5
Date: Thu, 3 Feb 2011 13:10:44 -0600
From: Kim.Donadio <@t> bhcpns.org
Subject: Re: [Histonet] Formaldehyde monitor
To: bob.nienhuis <@t> gmail.com
Cc: histonet <@t> lists.utsouthwestern.edu,
	histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
	<OF630DE85A.9E5052D3-ON8625782C.00693BE8-8625782C.00695A57 <@t> bhcpns.org>
Content-Type: text/plain;	charset="US-ASCII"

Call Advanced Chemical Sensors Inc @ 561-338-3116 

I think they are like $100 each. Last I looked anyway. 

Kim Donadio 
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996

Bob Nienhuis <bob.nienhuis <@t> gmail.com> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
02/03/2011 12:29 PM
Please respond to
bob.nienhuis <@t> gmail.com

To
histonet <@t> lists.utsouthwestern.edu
cc

Subject
[Histonet] Formaldehyde monitor

Anyone know of a cheap formaldehyde gas monitor or concentration test
method?

I found a couple of monitors on Google, but they seem to run abour $1500+

Bob
Sepulveda VA Med Center
Los Angeles
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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Message: 6
Date: Thu, 3 Feb 2011 13:12:42 -0600
From: <Wanda.Smith <@t> HCAhealthcare.com>
Subject: RE: [Histonet] Formaldehyde monitor
To: <bob.nienhuis <@t> gmail.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<9E2D36CE2D7CBA4A94D9B22E8328A3BA139EBA8097 <@t> NADCWPMSGCMS03.hca.corpad.net>

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Lab Safety Supply has the option to order the badges for formaldehyde and Xylene and when you order, you pre-pay for the analysis.  Formalin is $476.00 for 5 monitors.  Xylene is $259.00 for 4 monitors.  They email you a very nice report for your file, and it only takes about a week to 10 days.
Wanda

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bob Nienhuis
Sent: Thursday, February 03, 2011 1:29 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Formaldehyde monitor

Anyone know of a cheap formaldehyde gas monitor or concentration test
method?

I found a couple of monitors on Google, but they seem to run abour $1500+

Bob
Sepulveda VA Med Center
Los Angeles
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 7
Date: Thu, 3 Feb 2011 13:45:25 -0600
From: "Mike Pence" <mpence <@t> grhs.net>
Subject: RE: [Histonet] Consult accessioning question
To: <Wanda.Smith <@t> HCAhealthcare.com>, <Rcartun <@t> harthosp.org>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<661949901A768E4F9CC16D8AF8F2838C03974B1E <@t> is-e2k3.grhs.net>
Content-Type: text/plain;	charset="us-ascii"

Same for us. You need both DOS from office as well as date received.

Mike

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Wanda.Smith <@t> HCAhealthcare.com
Sent: Thursday, February 03, 2011 1:06 PM
To: Rcartun <@t> harthosp.org; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Consult accessioning question

In our LIS system we put in "specimen date" or DOS and we also have a
"Received date".  My understanding, for insurance/billing purposes, you
would need to include the original DOS, but you also need to be able to
document when you actually received the specimen. Just my 2 cents!!!
Wanda

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

This email and any files transmitted with it may contain PRIVILEGED or
CONFIDENTIAL information and may be read or used only by the intended
recipient. If you are not the intended recipient of the email or any of
its attachments, please be advised that you have received this email in
error and that any use, dissemination, distribution, forwarding,
printing, or copying of this email or any attached files is strictly
prohibited. If you have received this email in error, please immediately
purge it and all attachments and notify the sender by reply email or
contact the sender at the number listed.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Richard
Cartun
Sent: Thursday, February 03, 2011 1:43 PM
To: Histonet
Subject: [Histonet] Consult accessioning question

When accessioning a case for consultation, do you use the date on the
paperwork from the referring hospital/pathologist as the new
"Date-of-service"?  If so, what do you do when the paperwork gives a
date (say Friday) and then the specimen is not sent out until the
following Monday?  Thank you.

Richard 

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 8
Date: Thu, 03 Feb 2011 14:49:13 -0500
From: Merced M Leiker <leiker <@t> buffalo.edu>
Subject: Re: [Histonet] Off Topic: researchers very funny
To: Kimberly Tuttle <ktuttle <@t> umm.edu>,
	histonet <@t> lists.utsouthwestern.edu
Message-ID: <942FEC2A5A5B390A7D533E20 <@t> CDYwxp1931.ad.med.buffalo.edu>
Content-Type: text/plain; charset=us-ascii; format=flowed

yes our lab received it last week! And yes we've been posting it to 
Facebook as well (as someone else on Histonet commented!) If the lady in 
the video never gets her PhD, she may want to consider the entertainment 
industry!

--On Tuesday, February 01, 2011 10:29 PM -0500 Kimberly Tuttle 
<ktuttle <@t> umm.edu> wrote:

> If this is a repost I apologize :)
>
> http://www.youtube.com/watch?v=Fl4L4M8m4d0&feature=player_embedded
>
>
>
>
> This e-mail and any accompanying attachments may be privileged,
> confidential, contain protected health information about an identified
> patient or be otherwise protected from disclosure. State and federal law
> protect the confidentiality of this information. If the reader of this
> message is not the intended recipient; you are prohibited from using,
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>
> _______________________________________________
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> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.

------------------------------

Message: 9
Date: Thu, 3 Feb 2011 15:08:30 -0500
From: "Weems, Joyce" <JWeems <@t> sjha.org>
Subject: RE: [Histonet] Consult accessioning question
To: Richard Cartun <Rcartun <@t> harthosp.org>, Histonet
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<92AD9B20A6C38C4587A9FEBE3A30E164081DE5924C <@t> CHEXCMS10.one.ads.che.org>
Content-Type: text/plain; charset="us-ascii"

For Medicare, my understanding would be to use the DOS if less than 30 days, and to use the date pulled from archives if more than 30 days. At least that is the regs for additional tests. I think it would be the same for consult as well. 

Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Richard Cartun
Sent: Thursday, February 03, 2011 13:43
To: Histonet
Subject: [Histonet] Consult accessioning question

When accessioning a case for consultation, do you use the date on the paperwork from the referring hospital/pathologist as the new "Date-of-service"?  If so, what do you do when the paperwork gives a date (say Friday) and then the specimen is not sent out until the following Monday?  Thank you.

Richard 

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax

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Message: 10
Date: Thu, 3 Feb 2011 12:38:01 -0800
From: "Paula Lucas" <plucas <@t> biopath.org>
Subject: [Histonet] TTF-1/background staining
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <CCB966FDE20843769FC642A8B0D72012 <@t> biopath.local>
Content-Type: text/plain;	charset="us-ascii"

How can I eliminate the background or as you say, non-specific staining?

I'm no expert here, I admit, and so I'm asking for your help and
suggestions.  I have searched the Histonet archives, and a lot of what I'm
seeing deals with animal or rodent tissues, and a lot of it was confusing to
me.

To give you a little background info:

We use the Lab Vision stainer and the TTF-1 antibody we use is from Cell
Marque.  We were having issues with this marker from Lab Vision, so we
switched to TTF-1 a while ago.  We use the UltraVision LP detection kit from
Lab Vision. It's a polymer driven detection kit. 

The antibody is a ready to use, and we have the time set at 30 minutes.  

We were getting the background staining on a liver specimen last week, and
we also had this problem today on a lung case.  My doctor is getting
frustrated, and wants me to do something about this, and to repeat the TTF-1
stain on the lung, so if someone can give me some suggestions to try, I'm
ready to try them.

Thanks in advance,

Paula

Lab Manager

Bio-Path Medical Group

Fountain Valley, CA

------------------------------

Message: 11
Date: Thu, 3 Feb 2011 14:38:06 -0600
From: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>
Subject: [Histonet] Microwave processing
To: "'histonet <@t> lists.utsouthwestern.edu'"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<65365F35C0F2EF4D846EC3CA73E49C43FD0CF299E6 <@t> HPEMX3.HealthPartners.int>
Content-Type: text/plain; charset="us-ascii"

Does anyone know what the compliance is, if any, with JACHO or CAP regarding the pathologist doing a paper QC on each microwave run?  We have been having them sign a QC sheet that we hand in with each microwave run and our looking at ways to rid our area of some of the unnecessary paperwork!
Thanks!

Dorothy Webb

  ________________________________
This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited.

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Message: 12
Date: Thu, 3 Feb 2011 14:10:48 -0700
From: "Vanessa Avalos" <vavalos <@t> allergydermatology.com>
Subject: FW: [Histonet] Formaldehyde monitor
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000001cbc3e6$d4ae53c0$7e0afb40$@com>
Content-Type: text/plain;	charset="us-ascii"

We use Advanced Chemical Sensors.  Acsbadge.com is website. I also used to
use Surgipath.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bob Nienhuis
Sent: Thursday, February 03, 2011 11:29 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Formaldehyde monitor

Anyone know of a cheap formaldehyde gas monitor or concentration test
method?

I found a couple of monitors on Google, but they seem to run abour $1500+

Bob
Sepulveda VA Med Center
Los Angeles
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 13
Date: Thu, 3 Feb 2011 13:17:50 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Microwave processing
To: "'histonet <@t> lists.utsouthwestern.edu'"
	<histonet <@t> lists.utsouthwestern.edu>, 	Dorothy LWebb
	<Dorothy.L.Webb <@t> HealthPartners.Com>
Message-ID: <787421.30108.qm <@t> web65712.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Dorothy:
You should follow for MW processing the same procedure you follow for any "traditional" tissue processing.
What you have to document is that when you started to use the MW processing there was a documented QC to compare the results between the method you used and the new with the MW processor.
Having to sign a QC after each run is absolutely unnecessary if the initial QC was done.
Reni J.

--- On Thu, 2/3/11, Webb, Dorothy L <Dorothy.L.Webb <@t> HealthPartners.Com> wrote:

From: Webb, Dorothy L <Dorothy.L.Webb <@t> HealthPartners.Com>
Subject: [Histonet] Microwave processing
To: "'histonet <@t> lists.utsouthwestern.edu'" <histonet <@t> lists.utsouthwestern.edu>
Date: Thursday, February 3, 2011, 3:38 PM

Does anyone know what the compliance is, if any, with JACHO or CAP regarding the pathologist doing a paper QC on each microwave run?  We have been having them sign a QC sheet that we hand in with each microwave run and our looking at ways to rid our area of some of the unnecessary paperwork!
Thanks!

Dorothy Webb

  ________________________________
This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited.

If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0
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------------------------------

Message: 14
Date: Thu, 3 Feb 2011 13:57:22 -0800
From: James Watson <JWatson <@t> gnf.org>
Subject: [Histonet] Temporary histology position in San Diego Ca.
To: "'histonet <@t> lists.utsouthwestern.edu'"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<DD5C50737EA3284CA346C3767BFEABA903EB59FD17 <@t> MBX1.lj.gnf.org>
Content-Type: text/plain; charset="us-ascii"

GNF is currently seeking a temporary Scientific Associate to join the Histology

group, for the beginning of March 2011 through the end of July 2011. Perform

necropsies, tissue processing, slide sectioning, routine staining, special

staining, immunohistochemical staining and complex procedures necessary in

preparing specimens of animal tissue in a research environment.

Qualifications:

Associate's or B.S. degree in a biological science or completion of a NAACLS

accredited School of Histotechnology is required.  American Society of Clinical

Pathologist (ASCP) certification as a HT or HTL is required. Applicants must

demonstrate the potential ability to perform the essential functions of the job

as outlined in the position description. The employee must have 2-5 years of

experience in the Histology lab performing animal techniques, a wide variety of

manual histochemical and enzymatic staining, automated and manual

immunohistochemistry, and automated and manual in-situ hybridization.

Essential Functions:

1. Identifies significant tissue elements microscopically to determine quality

of staining.

2. Necropsy, fix, trim, process, and embed animal tissue for paraffin and

frozen sections.

3. Performs Microtomy on rotary microtome, cryostat, and be able to use a

sliding microtome.

4. Prepares dyes and solutions in order to perform routine, special, and

complex procedures.

5. Have experience in histochemical stains and enzymatic histochemical stains.

Be able to trouble shoot and correct problems with histochemical stains.  Have

experience in Immunohistochemical staining and other advanced histological

procedures.

6. Maintains lab work area by performing preventative maintenance on

instruments and equipment and keeping the work area clean and orderly.

7. Operate Slide scanning Instrumentation.

Interested candidates please send a copy of your CV with a summary of research

experience, and three references.

Job Code: JW10-013

Contact:E-mail: j... <@t> gnf.org<mailto:j... <@t> gnf.org>

James Watson HT  ASCP
Facilities Manager of Histology
GNF  Genomics Institute of the Novartis Research Foundation
Tel    858-332-4647
Fax   858-812-1915<mailto:858-812-1915jwatson <@t> gnf.org>
jwatson <@t> gnf.org

------------------------------

Message: 15
Date: Thu, 3 Feb 2011 16:02:22 -0600
From: Debra Siena <DSiena <@t> statlab.com>
Subject: RE: [Histonet] TTF-1/background staining
To: Paula Lucas <plucas <@t> biopath.org>,
	"histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B7F62D8163C233458A8CEC6920AEE00B2B9F8BD5CC <@t> statsbs>
Content-Type: text/plain; charset="iso-8859-1"

Hi Paula,

I am probably reading between the lines here but on your TTF protocol do you use a streptavidin biotin detection system and if so do you block for endogenous biotin, liver will have endogenous biotin where lung may not have as much?  thanks

Debbie Siena HT(ASCP)QIHC
Technical Manager | StatLab Medical Products
Direct: 972-436-1010  x229 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula Lucas
Sent: Thursday, February 03, 2011 2:38 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] TTF-1/background staining

How can I eliminate the background or as you say, non-specific staining?

I'm no expert here, I admit, and so I'm asking for your help and
suggestions.  I have searched the Histonet archives, and a lot of what I'm
seeing deals with animal or rodent tissues, and a lot of it was confusing to
me.

To give you a little background info:

We use the Lab Vision stainer and the TTF-1 antibody we use is from Cell
Marque.  We were having issues with this marker from Lab Vision, so we
switched to TTF-1 a while ago.  We use the UltraVision LP detection kit from
Lab Vision. It's a polymer driven detection kit. 

The antibody is a ready to use, and we have the time set at 30 minutes.  

We were getting the background staining on a liver specimen last week, and
we also had this problem today on a lung case.  My doctor is getting
frustrated, and wants me to do something about this, and to repeat the TTF-1
stain on the lung, so if someone can give me some suggestions to try, I'm
ready to try them.

Thanks in advance,

Paula

Lab Manager

Bio-Path Medical Group

Fountain Valley, CA

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 16
Date: Thu, 3 Feb 2011 17:54:25 -0500
From: "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com>
Subject: RE: [Histonet] Her2 Fixation Requirement 
To: "Weems, Joyce" <JWeems <@t> sjha.org>, "Paula Lucas"
	<plucas <@t> biopath.org>,	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<E78340C766A5284D999F5F5891DDF89014474AAD <@t> smcmail.somerset-healthcare.com>

Content-Type: text/plain;  charset="utf-8"

Does anyone have issues with inking the specimen after it has been placed in formalin?

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Weems,
Joyce
Sent: Wednesday, February 02, 2011 12:59 PM
To: Paula Lucas; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Her2 Fixation Requirement 

We have the nurse document the time of removal on the requisition. The next requisition update will have a spot for this. They have been very cooperative and do a good job. 

In addition, you must document the "cold ischmic time" - that is the time from removal until time in formalin. This is important when the specimen goes for xray or whatever. So there is a removal time, an into formalin time and an out of formalin time. 

Then if we don't have time allowed to meet the fixation time with the regular, it is put on a late processor, if we have one available, or it is held overnight. And if it has to come off on Sunday, we have a med tech remove it from the processor and it waits for us to come on Monday to embed it. 

The pathologists document all this time in the report. 

Best! J

Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula Lucas
Sent: Wednesday, February 02, 2011 12:11
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Her2 Fixation Requirement 

Hello histoland

I was just given the task to find a solution that is easy and will also comply with the CAP guideline for formalin fixation documentation, and so I started my research on the Histonet archives.  I found some good information, but was hoping to get more feedback. 

Would you mind sharing with me the actions you are taking to comply with the guideline?  

We are a private lab and we provide histology/pathology service for 2 hospitals and a few surgery centers.  We send our blocks to Genzyme for Her2, and we must document on their test order sheet how many hours the tissues have been fixed in formalin.

I'm assuming I will need to start keeping a log here, with documentation that shows what time the tissue was excised and placed in formalin from the OR, and then documentation that shows the time it was dissected and then placed in the tissue processor.  

The problem that I may come across is getting the OR nurse to document the time for us.  I don't know...maybe we need to put another sections on our requisition form, or maybe something on the formalin container itself for the nurse to write on.  It'll be a hassle at first but if I can get the hospitals lab director involved, I'm sure it will work itself out.

Anyway, if you wouldn't mind sharing some of your ideas, I would really appreciate it.

Paula Lucas
Lab Manager
Bio-Path Medical Group
Fountain Valley, CA

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------------------------------

Message: 17
Date: Thu, 03 Feb 2011 17:44:39 -0600
From: Nicole Cosenza <ncosenza <@t> siumed.edu>
Subject: [Histonet] Karnovsky and Roots stain
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4D4B3DE7.70203 <@t> siumed.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

I am looking into a project involving motor end plate staining. 
Literature that I've found continually references Karnovsky and Roots 
from the 60s.   However the papers are not supplying all the details.

Does anyone do AchE staining by this method on fresh frozen, unfixed 
tissue sections? If so, can I get a more detailed protocol  (fixation 
steps, washes, etc)?

------------------------------

Message: 18
Date: Fri, 04 Feb 2011 10:17:27 +1000
From: "Anthony Reilly" <Tony_Reilly <@t> health.qld.gov.au>
Subject: RE: [Histonet] TTF-1/background staining
To: "Paula Lucas" <plucas <@t> biopath.org>,
	"histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>, "Debra Siena"
	<DSiena <@t> statlab.com>
Message-ID: <4D4BD237.411C.0039.0 <@t> health.qld.gov.au>
Content-Type: text/plain; charset="us-ascii"

Hi Paula

I do not believe that RTU Abs really exist.  No company can produce an antibody that is suitable for use by all laboratories taking in to consideration how tissue can be handled differently from lab to lab.  I have used many RTU Abs which I have had to dilute to get optimal staining with Cam 5.2 a good example.  On the Bond I was using it at 1:25.  try titreing your Ab and see if that will reduce/eliminate your background.

regards
Tony

>>> Debra Siena <DSiena <@t> statlab.com> 4/02/2011 8:02 am >>>
Hi Paula,

I am probably reading between the lines here but on your TTF protocol do you use a streptavidin biotin detection system and if so do you block for endogenous biotin, liver will have endogenous biotin where lung may not have as much?  thanks

Debbie Siena HT(ASCP)QIHC
Technical Manager | StatLab Medical Products
Direct: 972-436-1010  x229 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula Lucas
Sent: Thursday, February 03, 2011 2:38 PM
To: histonet <@t> lists.utsouthwestern.edu 
Subject: [Histonet] TTF-1/background staining

How can I eliminate the background or as you say, non-specific staining?

I'm no expert here, I admit, and so I'm asking for your help and
suggestions.  I have searched the Histonet archives, and a lot of what I'm
seeing deals with animal or rodent tissues, and a lot of it was confusing to
me.

To give you a little background info:

We use the Lab Vision stainer and the TTF-1 antibody we use is from Cell
Marque.  We were having issues with this marker from Lab Vision, so we
switched to TTF-1 a while ago.  We use the UltraVision LP detection kit from
Lab Vision. It's a polymer driven detection kit. 

The antibody is a ready to use, and we have the time set at 30 minutes.  

We were getting the background staining on a liver specimen last week, and
we also had this problem today on a lung case.  My doctor is getting
frustrated, and wants me to do something about this, and to repeat the TTF-1
stain on the lung, so if someone can give me some suggestions to try, I'm
ready to try them.

Thanks in advance,

Paula

Lab Manager

Bio-Path Medical Group

Fountain Valley, CA

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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------------------------------

Message: 19
Date: Thu, 3 Feb 2011 18:39:15 -0800
From: Mark Tarango <marktarango <@t> gmail.com>
Subject: Re: [Histonet] TTF-1/background staining
To: Paula Lucas <plucas <@t> biopath.org>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<AANLkTik5xGfHe9LCN3rMe5rf7+yv9+5Ppte09QE1_YFq <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Hi Paula,

You mentioned the staining in a liver specimen using the cell marque
antibody against TTF-1.  The clone named 8G7G3/1 that cell marque sells is
known to stain liver cells and liver cancer.  Your pathologist might be
interested in knowing that.  Here is a reference you can give him:
http://www.nordiqc.org/Run-23-B5/Assessment/Assessment-TTF-1.htm   It also
lists another clone that doesn't cross react with liver.

Mark

On Thu, Feb 3, 2011 at 12:38 PM, Paula Lucas <plucas <@t> biopath.org> wrote:

> How can I eliminate the background or as you say, non-specific staining?
>
>
>
> I'm no expert here, I admit, and so I'm asking for your help and
> suggestions.  I have searched the Histonet archives, and a lot of what I'm
> seeing deals with animal or rodent tissues, and a lot of it was confusing
> to
> me.
>
>
>
> To give you a little background info:
>
> We use the Lab Vision stainer and the TTF-1 antibody we use is from Cell
> Marque.  We were having issues with this marker from Lab Vision, so we
> switched to TTF-1 a while ago.  We use the UltraVision LP detection kit
> from
> Lab Vision. It's a polymer driven detection kit.
>
>
>
> The antibody is a ready to use, and we have the time set at 30 minutes.
>
>
>
> We were getting the background staining on a liver specimen last week, and
> we also had this problem today on a lung case.  My doctor is getting
> frustrated, and wants me to do something about this, and to repeat the
> TTF-1
> stain on the lung, so if someone can give me some suggestions to try, I'm
> ready to try them.
>
>
>
> Thanks in advance,
>
> Paula
>
> Lab Manager
>
> Bio-Path Medical Group
>
> Fountain Valley, CA
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

------------------------------

Message: 20
Date: Fri, 4 Feb 2011 09:19:53 +0100
From: An Eerdekens <An.Eerdekens <@t> med.kuleuven.be>
Subject: [Histonet] thionin staining
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<85BD21DE1A6DE94D9A30A2F2C1D2328B3D547D231E <@t> ICTS-S-EXC4-CA.luna.kuleuven.be>

Content-Type: text/plain; charset="iso-8859-1"

Dear all,

I have problems with thionin staining. I am using this on paraffin slices of hypothalami. In the past, I never experienced problems, but the last times, the staining is washed off by going through the ethanol after staining (50%, 70%, 90%, 100%). I tried already a few protocols, but every time the same result.
Does anybody have experience with it?

regards

An Eerdekens

------------------------------

Message: 21
Date: Fri, 4 Feb 2011 02:19:39 -0800 (PST)
From: James Dooley <jdooley2008 <@t> yahoo.com>
Subject: [Histonet] Cre protocol-fresh frozen section
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <340255.99220.qm <@t> web45904.mail.sp1.yahoo.com>
Content-Type: text/plain; charset=us-ascii

Good Afternoon,

I would like to do fluoresent staining for Cre expression in thymus. Does anyone 
have protocol for doing this. I know Covance sells a Cre antibody.

Best regards,
James

------------------------------

Message: 22
Date: Fri, 4 Feb 2011 05:55:08 -0500 (EST)
From: erika <@t> mapslab.net
Subject: RE: [Histonet] CLIA classes?
To: "Sands, Jenn" <jsands <@t> innovishealth.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1296816908.91084963 <@t> 192.168.4.58>
Content-Type: text/plain;charset=UTF-8

I would be interested in this as well. B I supervise a dermpath lab and have for five years. B I have always passed with no deficiencies on my inspections. B The inspector always tells me that I document to much stuff. 

Thanks,
Erika

-----Original Message----- 
From: "Sands, Jenn" <jsands <@t> innovishealth.com> 
Sent: Thursday, February 3, 2011 11:02am 
To: indytreegers <@t> sbcglobal.net, Histonet <@t> lists.utsouthwestern.edu 
Subject: RE: [Histonet] CLIA classes?

I would be interested in this information as well.

Thank you.

Jenn Sands, CT(ASCP)cm, HTL(ASCP)cm

-----Original Message----- 
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of indytreegers <@t> sbcglobal.net 
Sent: Thursday, February 03, 2011 9:56 AM 
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CLIA classes? 

Hi all,

Does anyone know of a class available for learning about CLIA compliance and regulations, specifically for a Mohs/Dermpath lab? 

Thanks for any advice you can give me on this!

Lyn L. Treeger, B.S.HT(ASCP) 
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End of Histonet Digest, Vol 87, Issue 7
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