[Histonet] thionin staining

[An Eerdekens]

Dear all,

I have problems with thionin staining. I am using this on paraffin slices of hypothalami. In the past, I never experienced problems, but the last times, the staining is washed off by going through the ethanol after staining (50%, 70%, 90%, 100%). I tried already a few protocols, but every time the same result.
Does anybody have experience with it?

regards

An Eerdekens