[Histonet] CSF for cytology

Tony Henwood AnthonyH <@t> chw.edu.au
Wed Feb 2 20:06:38 CST 2011


CSF specimens usually contain very few cells, maybe a lymphocyte or two. Making a cell button (?cell block for Histo processing) would give disappointing results.

I would recommend cytocentrifugation (at least one air-dried DQ stained and one 95% ethanol-fixed PAP stained). You could even concentrate the cells using centrifugation prior to cytocentrifugation.

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Katelin Lester
Sent: Thursday, 3 February 2011 12:54 PM
To: histonet
Subject: [Histonet] CSF for cytology

Hi Histonet,
We are going to be receiving some cerebrospinal fluid as party of an MS Panel for cytology. Our pathologist would like me to make a cell button and stain it with H&E and Diff Quik, but I wanted to check with you guys and see if there are any other stains that I might do with this case.
Thank you for your help,
Katelin Lester, HTL(ASCP)
MedSurg Pathology Associates, Inc.

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