[Histonet] RE: Histonet Digest, Vol 87, Issue 4
Martin, Gary
gmartin <@t> marshallmedical.org
Wed Feb 2 13:16:20 CST 2011
We too are a small lab in California, and faced these same questions. We simply document all this in the report. For example; we know that our tissue comes out of formalin on the processor at 2330. We have the surgery staff document the time the specimen went into formalin. We then subtract that time form 2330 and have the hours in formalin. You are correct that it took a great deal of effort to see that surgery complied, but after they realized we were relentless with phone calls, things fell into place. Oh yes! On the weekend someone must come in to remove the tissue from the processor.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, February 02, 2011 10:01 AM
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Subject: Histonet Digest, Vol 87, Issue 4
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Today's Topics:
1. Re: Off Topic: researchers very funny (Paula Pierce)
2. Eosin stain for determining new bone old bone (Jacquitta Taylor)
3. TTF-1 Antibody (Joanne Clark)
4. Re: TTF-1 Antibody (BSullivan <@t> shorememorial.org)
5. Her2 Fixation Requirement (Paula Lucas)
6. Re: Her2 Fixation Requirement (BSullivan <@t> shorememorial.org)
7. RE: Her2 Fixation Requirement (Weems, Joyce)
----------------------------------------------------------------------
Message: 1
Date: Wed, 2 Feb 2011 05:49:57 -0800 (PST)
From: Paula Pierce <contact <@t> excaliburpathology.com>
Subject: Re: [Histonet] Off Topic: researchers very funny
To: Kimberly Tuttle <ktuttle <@t> umm.edu>, Histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <217328.82615.qm <@t> web1112.biz.mail.sk1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
This is hilarious. I got it on facebook yesterday and sent it to everyone.
Paula K. Pierce, AAS, BA, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
631 N Broadway
Moore, OK 73160
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com
________________________________
From: Kimberly Tuttle <ktuttle <@t> umm.edu>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Tue, February 1, 2011 9:29:25 PM
Subject: [Histonet] Off Topic: researchers very funny
If this is a repost I apologize :)
http://www.youtube.com/watch?v=Fl4L4M8m4d0&feature=player_embedded
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Message: 2
Date: Wed, 2 Feb 2011 09:13:40 -0600
From: Jacquitta Taylor <jacquitta <@t> earthlink.net>
Subject: [Histonet] Eosin stain for determining new bone old bone
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4C4B1F98-D6EF-45DF-A010-851CFFE11696 <@t> earthlink.net>
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Robin this is the protocol we use to showing new bone old bone.
Procedure for Eosin Y 0.6 % For Staining Bone Specimen
Chemicals
EosinY, Sigma E-4382,
100% Ethanol,
Phloxine B (Sigma P-4030)
Orange G(sodium salt-Sigma O-1625)
Preparation:
Stock 0.6% Eosin
Add 6g of eosin to 900ml 100% Ethanol and100 ml of H20; stir to dissolve.
Add 50 ml glacial acetic acid adjust PH to 4.6 and 5.0. The color of the solution will change from opaque green to clear red.
Stock 1% Phloxine B Solution:
Dissolve 1g of Phloxine B in 100 ml distilled water and filter to make a 1% solution.
Stock 2% Orange G Solution:
Dissolve 2 g of Orange G in 100ml distilled water and filter to make a 2% solution.
Working Solution Eosin 0.6%
Make a working solution by adding 6 ml of 1% Phloxine and 6 ml of 2% Orange G to 238 ml of 0.6% eosin.
Old bone dark orange.
New bone light orange.
Stain 15 to 30 seconds depending on what intensity you prefer.
------------------------------
Message: 3
Date: Wed, 2 Feb 2011 09:18:23 -0700
From: "Joanne Clark" <jclark <@t> pcnm.com>
Subject: [Histonet] TTF-1 Antibody
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39C01486A3D <@t> mail.pcnm.com>
Content-Type: text/plain; charset="us-ascii"
Good Morning, we are using TTF-1 antibody from Cell Marque (clone
8G7G3/1 mouse monoclonal) on a DAKO autostainer with an LSAB+ platform.
We do heat retrieval in the DAKO pascal pressure cooker using Cell
Marques Trilogy retrieval solution. We do a lower temp for a longer
period of time during the retrieval. I incubate the primary antibody
for 1 hour and I still have problems getting the marker to work with any
consistency.
Where do the rest of you doing this marker get your antibody from and do
you have problems getting it to work consistently? Any and all feedback
would be appreciated, I'm getting really frustrated.
Thanks
Joanne Clark, HT
Histology Supervisor
Pathology Consultants of New Mexico
------------------------------
Message: 4
Date: Wed, 2 Feb 2011 11:31:51 -0500
From: BSullivan <@t> shorememorial.org
Subject: Re: [Histonet] TTF-1 Antibody
To: "Joanne Clark" <jclark <@t> pcnm.com>
Cc: histonet <@t> lists.utsouthwestern.edu,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OF7948C343.994CF3FC-ON8525782B.005AAAF5-8525782B.005B1A87 <@t> shorememorial.org>
Content-Type: text/plain; charset=US-ASCII
We purchase out TTF-1 antibody from Ventana Medical and use it on their
Benchmark XT stainer. I do believe they might purchase this from Cell
Marque but I could be wrong. Anyway, we have no problem with this stain.
Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore Memorial Hospital
609-653-3590
Speak only well of people and you need never whisper
"Joanne Clark"
<jclark <@t> pcnm.com>
Sent by: To
histonet-bounces@ <histonet <@t> lists.utsouthwestern.edu>
lists.utsouthwest cc
ern.edu
Subject
[Histonet] TTF-1 Antibody
02/02/2011 11:18
AM
Good Morning, we are using TTF-1 antibody from Cell Marque (clone
8G7G3/1 mouse monoclonal) on a DAKO autostainer with an LSAB+ platform.
We do heat retrieval in the DAKO pascal pressure cooker using Cell
Marques Trilogy retrieval solution. We do a lower temp for a longer
period of time during the retrieval. I incubate the primary antibody
for 1 hour and I still have problems getting the marker to work with any
consistency.
Where do the rest of you doing this marker get your antibody from and do
you have problems getting it to work consistently? Any and all feedback
would be appreciated, I'm getting really frustrated.
Thanks
Joanne Clark, HT
Histology Supervisor
Pathology Consultants of New Mexico
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------------------------------
Message: 5
Date: Wed, 2 Feb 2011 09:11:10 -0800
From: "Paula Lucas" <plucas <@t> biopath.org>
Subject: [Histonet] Her2 Fixation Requirement
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <2FB3F34C3F1047489B7F2E0A9E3E96E9 <@t> biopath.local>
Content-Type: text/plain; charset="us-ascii"
Hello histoland
I was just given the task to find a solution that is easy and will also
comply with the CAP guideline for formalin fixation documentation, and so I
started my research on the Histonet archives. I found some good
information, but was hoping to get more feedback.
Would you mind sharing with me the actions you are taking to comply with the
guideline?
We are a private lab and we provide histology/pathology service for 2
hospitals and a few surgery centers. We send our blocks to Genzyme for
Her2, and we must document on their test order sheet how many hours the
tissues have been fixed in formalin.
I'm assuming I will need to start keeping a log here, with documentation
that shows what time the tissue was excised and placed in formalin from the
OR, and then documentation that shows the time it was dissected and then
placed in the tissue processor.
The problem that I may come across is getting the OR nurse to document the
time for us. I don't know...maybe we need to put another sections on our
requisition form, or maybe something on the formalin container itself for
the nurse to write on. It'll be a hassle at first but if I can get the
hospitals lab director involved, I'm sure it will work itself out.
Anyway, if you wouldn't mind sharing some of your ideas, I would really
appreciate it.
Paula Lucas
Lab Manager
Bio-Path Medical Group
Fountain Valley, CA
------------------------------
Message: 6
Date: Wed, 2 Feb 2011 12:27:54 -0500
From: BSullivan <@t> shorememorial.org
Subject: Re: [Histonet] Her2 Fixation Requirement
To: "Paula Lucas" <plucas <@t> biopath.org>
Cc: histonet <@t> lists.utsouthwestern.edu,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OF9C001E71.89F976F8-ON8525782B.005E6D4E-8525782B.00603CD3 <@t> shorememorial.org>
Content-Type: text/plain; charset=US-ASCII
We receive our lumpectomies and total breasts fresh. They are immediately
put in 10 % formalin if no frozen is required and that time is noted on our
requisition. Our processing time for formalin and the fixation time is made
part of the final report. If we receive a breast biopsy specimen we have
asked that they write the time on the requistion that the specimen was
placed in 10 % formalin. We set up a procedure that states this.
Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore Memorial Hospital
609-653-3590
Speak only well of people and you need never whisper
"Paula Lucas"
<plucas <@t> biopath.o
rg> To
Sent by: <histonet <@t> lists.utsouthwestern.edu>
histonet-bounces@ cc
lists.utsouthwest
ern.edu Subject
[Histonet] Her2 Fixation
Requirement
02/02/2011 12:11
PM
Hello histoland
I was just given the task to find a solution that is easy and will also
comply with the CAP guideline for formalin fixation documentation, and so I
started my research on the Histonet archives. I found some good
information, but was hoping to get more feedback.
Would you mind sharing with me the actions you are taking to comply with
the
guideline?
We are a private lab and we provide histology/pathology service for 2
hospitals and a few surgery centers. We send our blocks to Genzyme for
Her2, and we must document on their test order sheet how many hours the
tissues have been fixed in formalin.
I'm assuming I will need to start keeping a log here, with documentation
that shows what time the tissue was excised and placed in formalin from the
OR, and then documentation that shows the time it was dissected and then
placed in the tissue processor.
The problem that I may come across is getting the OR nurse to document the
time for us. I don't know...maybe we need to put another sections on our
requisition form, or maybe something on the formalin container itself for
the nurse to write on. It'll be a hassle at first but if I can get the
hospitals lab director involved, I'm sure it will work itself out.
Anyway, if you wouldn't mind sharing some of your ideas, I would really
appreciate it.
Paula Lucas
Lab Manager
Bio-Path Medical Group
Fountain Valley, CA
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------------------------------
Message: 7
Date: Wed, 2 Feb 2011 12:58:36 -0500
From: "Weems, Joyce" <JWeems <@t> sjha.org>
Subject: RE: [Histonet] Her2 Fixation Requirement
To: Paula Lucas <plucas <@t> biopath.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<92AD9B20A6C38C4587A9FEBE3A30E164081DE58F2F <@t> CHEXCMS10.one.ads.che.org>
Content-Type: text/plain; charset="us-ascii"
We have the nurse document the time of removal on the requisition. The next requisition update will have a spot for this. They have been very cooperative and do a good job.
In addition, you must document the "cold ischmic time" - that is the time from removal until time in formalin. This is important when the specimen goes for xray or whatever. So there is a removal time, an into formalin time and an out of formalin time.
Then if we don't have time allowed to meet the fixation time with the regular, it is put on a late processor, if we have one available, or it is held overnight. And if it has to come off on Sunday, we have a med tech remove it from the processor and it waits for us to come on Monday to embed it.
The pathologists document all this time in the report.
Best! J
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula Lucas
Sent: Wednesday, February 02, 2011 12:11
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Her2 Fixation Requirement
Hello histoland
I was just given the task to find a solution that is easy and will also comply with the CAP guideline for formalin fixation documentation, and so I started my research on the Histonet archives. I found some good information, but was hoping to get more feedback.
Would you mind sharing with me the actions you are taking to comply with the guideline?
We are a private lab and we provide histology/pathology service for 2 hospitals and a few surgery centers. We send our blocks to Genzyme for Her2, and we must document on their test order sheet how many hours the tissues have been fixed in formalin.
I'm assuming I will need to start keeping a log here, with documentation that shows what time the tissue was excised and placed in formalin from the OR, and then documentation that shows the time it was dissected and then placed in the tissue processor.
The problem that I may come across is getting the OR nurse to document the time for us. I don't know...maybe we need to put another sections on our requisition form, or maybe something on the formalin container itself for the nurse to write on. It'll be a hassle at first but if I can get the hospitals lab director involved, I'm sure it will work itself out.
Anyway, if you wouldn't mind sharing some of your ideas, I would really appreciate it.
Paula Lucas
Lab Manager
Bio-Path Medical Group
Fountain Valley, CA
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