[Histonet] calibration of pH meter

[Kuhnla, Melissa]

Is anyone aware of any actual standards?  I routinely calibrate the ph
meter each time we use it. I calibrate, and then take the reading of the
newly made buffer.  Is this sufficient? I recently had my compliance
officer tell me the this was not sufficient. She thinks we need to
calibrate, take a reading of a solution with a known ph, and then take
the reading of the new solution.  What is everyone doing??????? Thank
you,
Melissa

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jan
Shivers
Sent: Tuesday, February 01, 2011 3:13 PM
To: Susan Bachus; histonet; Akemi Allison
Subject: Re: [Histonet] calibration of pH meter

For our equipment quality control, we're required to record every single

calibration value each time we calibrate/verify pHs.  The document is 
reviewed regularly to see if there are any abnormal out-of-range pH-ing 
trends starting to happen.  We also aren't allowed to put the probe into
the 
various pH buffer bottles, but need to pour buffers into separate
beakers 
and use them once, then toss.

Jan Shivers


----- Original Message ----- 
From: "Susan Bachus" <susanbachus <@t> verizon.net>
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>; "Akemi Allison" 
<akemiat3377 <@t> yahoo.com>
Sent: Tuesday, February 01, 2011 1:49 PM
Subject: Re: [Histonet] calibration of pH meter


> This may sound simplistic, but it works for us:   whenever I need to
use 
> pH something, I quickly first dunk the electrode in the buffers above
& 
> below what I'm pHing (rinsing after each dunk, of course)--if the
meter 
> reads accurately I proceed to measure, if it's off by more than a few
% I 
> recalibrate before measuring.    It's true that a new electrode can
stay 
> well calibrated for long periods.    Susan
> ----- Original Message ----- 
> From: "Jan Shivers" <shive003 <@t> umn.edu>
> To: "histonet" <histonet <@t> lists.utsouthwestern.edu>; "Akemi Allison" 
> <akemiat3377 <@t> yahoo.com>
> Sent: Tuesday, February 01, 2011 1:36 PM
> Subject: Re: [Histonet] calibration of pH meter
>
>
>> We calibrate our pH meter every day that it's used, which can be
daily 
>> during heavy workload weeks, using multiple calibration buffers.
>>
>> Jan Shivers
>> UMN Vet Diag Lab
>>
>> ----- Original Message ----- 
>> From: "Akemi Allison" <akemiat3377 <@t> yahoo.com>
>> To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
>> Sent: Tuesday, February 01, 2011 7:54 AM
>> Subject: [Histonet] calibration of pH meter
>>
>>
>>> Good morning everyone,
>>>
>>> I am curious how frequently you all calibrate your pH meter, 
>>> particularly when you make up solutions for muscle bx's?  Do you do
it 
>>> daily, weekly, or monthly?  I am trying to come up with an
acceptable 
>>> standard in a clinical histology lab, verses a biotech  lab, which 
>>> makes-up large quantities of reagents on a daily basis.
>>>
>>> Thank you in advance for your information,
>>> Akemi
>>>
>>>
>>> Akemi Allison BS, HT (ASCP) HTL
>>> Director
>>> Phoenix Lab Consulting
>>> Tele: 408.335.9994
>>> E-Mail: akemiat3377 <@t> yahoo.com
>>>
>>> _______________________________________________
>>> Histonet mailing list
>>> Histonet <@t> lists.utsouthwestern.edu
>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>>
>>
>>
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>
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