[Histonet] stains for visualizing new bone growth

Liz Chlipala liz <@t> premierlab.com
Tue Feb 1 14:23:00 CST 2011


I'm not sure how accurate this is, but we do a lot of massons trichrome
staining in rat muscle pouch studies (they inject into the muscle pouch
something that will induce bone formation) and we have noticed that new
bone formation has a tendency to stain blue rather than red.  You could
also try a Goldners trichrome too.  That is supposed to stain new bone


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Robin
Sent: Tuesday, February 01, 2011 12:45 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] stains for visualizing new bone growth

Does anyone know of a good stain to use to clearly show new bone growth
other than von Kossa stain?

Would appreciate any suggestions anyone might have.


Thank you,



Robin R. Dean, Ph.D.

Senior Scientist & Study Director

Comparative Biosciences, Inc.

786 Lucerne Dr.

Sunnyvale, CA

(408) 738-8060

robin_dean <@t> compbio.com


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