[Histonet] RE: IHC on Bones

[McMahon, Loralee A]

I have place the deparaffinized slides into 10% neutral buffered formalin for about 30 minutes before the heat retrieval.  Somehow this seems to work 
You can also lower the temperature of your retrieval and lengthen the time. 

Good Luck. 

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of McCabe, Sara J. [SJMccabe <@t> drmc.org]
Sent: Friday, December 30, 2011 1:02 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC on Bones

Hello Histonet,

Wondering what those of you that do IHC on bones do to ensure that the tissue remains on the slide during processing?

Thanks,
Sara McCabe HT(ASCP)
DuBois Regional Medical Center

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