[Histonet] RE: IHC on Bones

[Elizabeth Chlipala]

Sara

If you can you want to stick with an enzyme digestion such as proteinase K or another enzyme.  If the antibody requires HIER and will not work with enzyme digestion then modify your retreival time and temp, we use 70C for 2 hours and find that this works for most antibodies.  Some antibodies that require HIER may not work with the longer/lower temp methods.

Good Luck

Liz

________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of McCabe, Sara J. [SJMccabe <@t> drmc.org]
Sent: Friday, December 30, 2011 11:02 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC on Bones

Hello Histonet,

Wondering what those of you that do IHC on bones do to ensure that the tissue remains on the slide during processing?

Thanks,
Sara McCabe HT(ASCP)
DuBois Regional Medical Center

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