[Histonet] Re: Histonet Digest, Vol 97, Issue 32
Madeleine Huey
madeleinehuey <@t> gmail.com
Fri Dec 30 00:31:20 CST 2011
Gui,
I don't see any problem with the protocol.
Here's what I would do before waisting my time on the ICC staining.
I check the viability of the cells before staining, because many thing
can go wrong with the primary culture (my guest it's not cell line, am
I right?).
- To do this, at the end of culturing, I rinse off the medium with
pre-chilled PBS without any ionic detergent (ie. Triton, Tween 20,
& etc).
- Add DAPI/PBS (your working concentration is fine) for 5 min @ RT.
- Wash off excess DAPI with PBS x3.
- Take the cells & check under the fluorescent microscope if the
NUCLEI are in good condition (with proper filter). They should be the
same morphology as your 1st day culture after they adherent.
** If they look the same as your ICC, then that mean it's not the
protocol problem. It's your tissue culture problem. Primary rodent
brain culture are Not easy to culture.
Please let me know if my suggestion are helping or not.
Good Luck!
Madeleine Huey BS, HTL/QIHC (ASCP)
Supervisor-Pathology (IPOX & Histology)
El Camino Hospitial
madeleine_h <@t> elcaminohospital.org
On Thu, Dec 29, 2011 at 10:00 AM,
<histonet-request <@t> lists.utsouthwestern.edu> wrote:
> Send Histonet mailing list submissions to
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>
> Today's Topics:
>
> 1. pap supply processing (Gale Limron)
> 2. CPT CODE 88106 and 88104 (Tunde Ajibade)
> 3. RE: Histonet Digest, Vol 97, Issue 31 (Goodwin, Diana)
> 4. Shatter effect on specimens (Jimmy Lofton)
> 5. Histologist needed UAMS Little Rock AR (Marcum, Pamela A)
> 6. Saturday Coverage Orange County California (Paula Lucas)
> 7. RE: Shatter effect on specimens (joelle weaver)
> 8. C4d immunofluorescent positive controls (Martha Ward-Pathology)
> 9. RE: C4d immunofluorescent positive controls (Tunde Ajibade)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Wed, 28 Dec 2011 13:06:13 -0500
> From: Gale Limron <GaleL <@t> unionhospital.org>
> Subject: [Histonet] pap supply processing
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <D7B0000361AAAF4A8895E5918634850A04D98F1DDF <@t> uhexg-3.unionhospital.org>
> Content-Type: text/plain; charset="us-ascii"
>
> Happy Holidays!
> Can anyone tell me what their current per-test pricing is for ThinPrep pap supplies? I'm putting together a study to present numbers to possibly bring our Gyn Cytology back in-house.
> Thank you,
> Gale
>
> Gale Limron CT,HT (ASCP)
> Histology Supervisor
> Union Hospital
> 659 Boulevard
> Dover, Ohio 44622
> 330-343-3311 ext 2562
>
>
>
> This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery.
>
> ------------------------------
>
> Message: 2
> Date: Wed, 28 Dec 2011 12:46:50 -0600
> From: Tunde Ajibade <tajibade <@t> echd.org>
> Subject: [Histonet] CPT CODE 88106 and 88104
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>, Tunde Ajibade <tajibade <@t> echd.org>
> Message-ID:
> <ABF9E8674BBE5448920911351AC6D3E50208FB578E <@t> MCHMAIL.echd.org>
> Content-Type: text/plain; charset="us-ascii"
>
> Hello everyone,
> I need more information about the following CPT:
> CPT 88106-Cytopathology, fluids, washing or brushings, except cervical or vaginal: SIMPLE FILTER METHOD with interpretation
> CPT 88104 -Cytopathology, fluids, washing or brushings, except cervical or vaginal: smears with interpretation.
> My question is that, what is the SIMPLE FILTER METHOD means for CPT 88106? Does it means making of thin prep from the specimen? Which one is more appropriate to use? I need help. Thanks
>
> Tunde Ajibade BS, HTL(ASCP)QIHC
>
>
>
>
> CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents.
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> ------------------------------
>
> Message: 3
> Date: Wed, 28 Dec 2011 13:53:11 -0500
> From: "Goodwin, Diana" <dgoodwin <@t> rwjuhh.edu>
> Subject: [Histonet] RE: Histonet Digest, Vol 97, Issue 31
> To: "'histonet <@t> lists.utsouthwestern.edu'"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <09411E0112A96A459D8D5FBDAB9C15C72480DAE383 <@t> HAMEXMBA.rwjham.local>
> Content-Type: text/plain; charset="us-ascii"
>
> RE: standard for sm bx microtomy (Sharon Allen)
>
> Hi, Sharon.
>
> We are cutting 2 H&E levels per block, with 1 extra slide and 1 special stain slide in between - the special being dependent on the type of tissue-HP Giemsa for upper GI, PAS/AB for esophagus, Mucin for lung, etc.
>
> Hope this helps.
>
> Diana G. Goodwin, BS, HT(ASCP)QIHC
> Department of Pathology
> Robert Wood Johnson University Hospital at Hamilton
> One Hamilton Health Place
> Hamilton, NJ 08690
> Ph: 609.631.6996
> Email: dgoodwin <@t> rwjuhh.edu
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
> Sent: Wednesday, December 28, 2011 1:04 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: Histonet Digest, Vol 97, Issue 31
>
> Send Histonet mailing list submissions to
> histonet <@t> lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
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> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
> 1. Re: Histonet Digest, Vol 97, Issue 30 (Thomas "Tom" Ehlers)
> 2. Re: immuno stainers (Blazek, Linda)
> 3. good protocol for ICC in neurons (Guillermo Palchik)
> 4. Re: immuno stainers (Karen Lahti)
> 5. gel pad for microtome vibration (Nancy Schmitt)
> 6. FW: Quality Modules (Sharon Allen)
> 7. FW: standard for sm bx microtomy (Sharon Allen)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Tue, 27 Dec 2011 14:56:54 -0500
> From: "Thomas \"Tom\" Ehlers" <tehlers818 <@t> gmail.com>
> Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 30
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <CAHw4HRSDjPyAmxhrXe-Gf8YgU8ozANYuz5YFPDF+NqsD5LHe4w <@t> mail.gmail.com>
> Content-Type: text/plain; charset=windows-1252
>
> Hi Cindy,
>
> What's the name of the new Dako stainer that you demo'd?
>
> On Tue, Dec 27, 2011 at 1:00 PM,
> <histonet-request <@t> lists.utsouthwestern.edu>wrote:
>
>> Send Histonet mailing list submissions to
>> histonet <@t> lists.utsouthwestern.edu
>>
>> To subscribe or unsubscribe via the World Wide Web, visit
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> or, via email, send a message with subject or body 'help' to
>> histonet-request <@t> lists.utsouthwestern.edu
>>
>> You can reach the person managing the list at
>> histonet-owner <@t> lists.utsouthwestern.edu
>>
>> When replying, please edit your Subject line so it is more specific
>> than "Re: Contents of Histonet digest..."
>>
>>
>> Today's Topics:
>>
>> 1. RE: HSV1 and HSV2 (Hoekert, W.E.J.)
>> 2. RELIA HOT Histology Job Alert. 12/27/2011 Great Opportunity
>> for a histology tech in Virginia. (Pam Barker)
>> 3. immuno stainers (Cynthia Pyse)
>>
>>
>> ----------------------------------------------------------------------
>>
>> Message: 1
>> Date: Tue, 27 Dec 2011 10:38:20 +0100
>> From: "Hoekert, W.E.J." <W.E.J.Hoekert <@t> olvg.nl>
>> Subject: RE: [Histonet] HSV1 and HSV2
>> To: "Evans, Andria B" <aevans3 <@t> lghealth.org>,
>> <histonet <@t> lists.utsouthwestern.edu>
>> Message-ID: <1190CB05C44B13409483514729C2FC3601F84207 <@t> PAIT42.olvg.nl>
>> Content-Type: text/plain; charset="iso-8859-1"
>>
>> For the immunologic pAB cocktail we are using Protease 1 (4 min) and ab
>> incubation of 32 min. The only problem is that mast cells are also
>> staining, it is a side effect of the protease 1.
>>
>> (AB concentration 1:400)
>>
>> Willem Hoekert
>> OLVG Amsterdam
>> The Netherlands
>>
>>
>>
>>
>> ________________________________
>>
>> Van: histonet-bounces <@t> lists.utsouthwestern.edu namens Evans, Andria B
>> Verzonden: vr 23-12-2011 18:48
>> Aan: histonet <@t> lists.utsouthwestern.edu
>> Onderwerp: [Histonet] HSV1 and HSV2
>>
>>
>>
>> I am currently having issues with our HSV1 and HSV2 staining tissue
>> components that it shouldn't be/background. We are running Ventana
>> Benchmark XTs and Ultras. Using Dako's polyclonal rabbit concentrate.
>> Does anyone out there in histoland have a concentration with a protocol
>> that works and looks clean?
>>
>> Thank you for your help!
>>
>> Andria B Evans HTL(ASCP)CM
>> This email was sent securely from the LGHealth Email Service
>>
>> Confidentiality Notice:
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>> of intended recipient(s) and may contain confidential and
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>> _______________________________________________
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>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>>
>> Disclaimer:
>>
>> Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n).
>> Verstrekking aan en gebruik door anderen dan geadresseerden is niet
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>>
>>
>>
>> ------------------------------
>>
>> Message: 2
>> Date: Tue, 27 Dec 2011 11:26:52 -0500
>> From: "Pam Barker" <relia1 <@t> earthlink.net>
>> Subject: [Histonet] RELIA HOT Histology Job Alert. 12/27/2011 Great
>> Opportunity for a histology tech in Virginia.
>> To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
>> Message-ID: <2AD338F5D60F42969C3852F762D09410 <@t> ownerf1abaad51>
>> Content-Type: text/plain; charset="iso-8859-1"
>>
>> Hi Histonetters,
>> Seasons Greetings!!
>> I hope everyone enjoyed a wonderful holiday weekend. I have a brand new
>> histology job that I am pretty excited about and I wanted to take a
>> minute and share the info with you:
>>
>> RELIA Solutions the nation?s only recruiting firm dedicated to the
>> nationwide permanent placement of histology professionals is assisting a
>> leading lab in Roanoke, VA in their search for a histology tech. ASCP
>> HT/HTL and 3 years experience preferred. IHC is a plus. This is a day
>> shift (early morning) M-F full time permanent position. My client
>> offers a great salary and excellent benefits. For more information
>> please contact Pam Barker at <mailto:relia1 <@t> earthlink.net>
>> relia1 <@t> earthlink.net or toll free at 866-607-3542
>>
>> If you or anyone you know might be interested in hearing more about this
>> opportunity please contact me. I can be reached at
>> <mailto:relia1 <@t> earthlink.net> relia1 <@t> earthlink.net or toll free at
>> 866-607-3542.
>>
>> Thanks-Pam
>>
>>
>> Thank You!
>>
>>
>>
>> Pam Barker
>> President
>> RELIA
>> Specialists in Allied Healthcare Recruiting
>> 5703 Red Bug Lake Road #330
>> Winter Springs, FL 32708-4969
>> Phone: (407)657-2027
>> Cell: (407)353-5070
>> FAX: (407)678-2788
>> E-mail: <mailto:relia1 <@t> earthlink.net> relia1 <@t> earthlink.net
>> <http://www.facebook.comPamBarkerRELIA> www.facebook.comPamBarkerRELIA
>> <http://www.linkedin.com/reliasolutions>
>> www.linkedin.com/reliasolutions
>> <http://www.myspace.com/pamatrelia> www.myspace.com/pamatrelia
>> <http://www.twitter.com/pamatrelia> www.twitter.com/pamatrelia
>>
>>
>>
>> ------------------------------
>>
>> Message: 3
>> Date: Tue, 27 Dec 2011 11:49:50 -0500
>> From: "Cynthia Pyse" <cpyse <@t> x-celllab.com>
>> Subject: [Histonet] immuno stainers
>> To: <histonet <@t> lists.utsouthwestern.edu>
>> Message-ID: <003e01ccc4b7$8cb51a40$a61f4ec0$@com>
>> Content-Type: text/plain; charset="us-ascii"
>>
>> Hello Histonetters
>>
>> Happy Tuesday. I hope everyone had a Merry Christmas. It is amazing how
>> times flies when you have time off. Now it's back to the old laboratory
>> grind.
>>
>> We are in the market for a new immuno stainer. I currently use a Dako Link,
>> which I am very pleased with. I have demoed the new Dako with great
>> results,
>> unfortunately we need 2 stainers and the pricing maybe a little out of our
>> range.
>>
>> Is anyone using the Biocare Intellipath? What are the pro's and con's? How
>> is their service? I currently use their detection system, Mach 4, but the
>> majority of my antibodies are purchased from Dako. Any input on the daily
>> use of the Intellipath from current users would be appreciated.
>>
>> Thanks in advance for the input
>>
>> Cindy
>>
>>
>>
>> Cindy Pyse, CLT, HT (ASCP)
>>
>> Laboratory Manager
>>
>> X-Cell Laboratories
>>
>> e-mail cpyse <@t> x-celllab.com
>>
>>
>>
>>
>>
>> ------------------------------
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>> End of Histonet Digest, Vol 97, Issue 30
>> ****************************************
>>
>
>
> ------------------------------
>
> Message: 2
> Date: Tue, 27 Dec 2011 14:58:46 -0500
> From: "Blazek, Linda" <lblazek <@t> digestivespecialists.com>
> Subject: Re: [Histonet] immuno stainers
> To: Cynthia Pyse <cpyse <@t> x-celllab.com>
> Cc: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <F7B33547-74AA-4071-B193-3C222C05A6DA <@t> digestivespecialists.com>
> Content-Type: text/plain; charset="us-ascii"
>
> I have the Intellipath from BioCare. I love it. Service is spectacular. It's very user friendly. You do have to be consistent with your cleaning protocol. In the past 3-4 years I have only had 1 day of down time. The company is very proactive when it comes to service. You couldn't ask for better a better company to work with in both sales or service. If you want to contact me directly feel.
> Linda Blazek
> GI Pathology
> 937 396-2623
>
> Sent from my iPhone
>
> On Dec 27, 2011, at 11:51 AM, "Cynthia Pyse" <cpyse <@t> x-celllab.com> wrote:
>
>> Hello Histonetters
>>
>> Happy Tuesday. I hope everyone had a Merry Christmas. It is amazing how
>> times flies when you have time off. Now it's back to the old laboratory
>> grind.
>>
>> We are in the market for a new immuno stainer. I currently use a Dako Link,
>> which I am very pleased with. I have demoed the new Dako with great results,
>> unfortunately we need 2 stainers and the pricing maybe a little out of our
>> range.
>>
>> Is anyone using the Biocare Intellipath? What are the pro's and con's? How
>> is their service? I currently use their detection system, Mach 4, but the
>> majority of my antibodies are purchased from Dako. Any input on the daily
>> use of the Intellipath from current users would be appreciated.
>>
>> Thanks in advance for the input
>>
>> Cindy
>>
>>
>>
>> Cindy Pyse, CLT, HT (ASCP)
>>
>> Laboratory Manager
>>
>> X-Cell Laboratories
>>
>> e-mail cpyse <@t> x-celllab.com
>>
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 3
> Date: Tue, 27 Dec 2011 16:32:06 -0500
> From: Guillermo Palchik <gp62 <@t> georgetown.edu>
> Subject: [Histonet] good protocol for ICC in neurons
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <80DAFD47-401A-4FAF-AB05-32EC6AA81364 <@t> georgetown.edu>
> Content-Type: text/plain; charset=us-ascii
>
> Dear histonetters,
>
> I am trying to do some ICC on nuclei of rat primary neurons that have been grown for 2 weeks on coverslips (seeded at 75,000 cells/ml). So far I have followed the protocol that a (long since gone) student in the lab used, but I have been unsuccessful. My nuclei end up looking like raisins....
> The protocol that the student used is unlike anything I have seen:
> Wash with cold PBS 2X 5 min
>
> Rinse briefly with cold methanol
>
> Fix in methanol at -20C for 20 minutes
>
> Wash in cold PBS 2 X 10 min
>
> Block in 5% NGS / 0.05% Triton-X for 6 hours at 4C
>
> Add Primary antibodies diluted in Blocking Solution overnight @ 4C
>
> Wash in cold Wash Solution (2% NGS/0.05% Triton-X) 3 X 10 min
>
> Add Secondary Antibodies diluted in Blocking Solution for 1.5 hours at RT in dark
>
> Wash in Wash Solution 3 X 20 min in dark
>
>
> Wash in PBS 3 X 10 min in dark
>
> Add DAPI (1:10,000 in PBS) for 5 minutes in dark
>
> Wash in ddH2O 3 X 10 min in dark
>
> Mount with Fluoro-Gel with Tris
>
> Store @ 4C in dark until dry
>
>
>
> First, after a methanol fixation of 20 minutes at -20C the protocol calls for blocking and permeabilizing the cells for 6 hours!
>
> Second, it keeps washing with this washing solution that contains triton.
>
> As I said, I have followed this protocol to the T and I keep getting horrible looking, shriveled cells and nuclei...
> The question I have is, does the protocol look okay and maybe it is me, or should I change specific parameters of it?
>
> Does anybody have a good working protocol that could share with me? It would be greatly appreciated...
> Thanks
> Gil
>
> --
> Guillermo Palchik
> Ph.D. Candidate - Interdisciplinary Program in Neuroscience
> Georgetown University Medical Center
> Research Building Room W 217
> 3970 Reservoir Rd. NW, Washington, DC 20007
> Lab: 202-687-7825
>
>
>
> ------------------------------
>
> Message: 4
> Date: Tue, 27 Dec 2011 19:34:36 -0700
> From: Karen Lahti <karen <@t> gateslinger.com>
> Subject: Re: [Histonet] immuno stainers
> To: "Blazek, Linda" <lblazek <@t> digestivespecialists.com>
> Cc: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <BEBB5A0E-E0AF-417B-8632-01E5CFF97858 <@t> gateslinger.com>
> Content-Type: text/plain; charset=us-ascii
>
> We have the IntelliPath as well. I have the same sentiments as Linda regarding all aspects of Biocare and the instrument. We use their antibodies and detection. The company as a whole is excellent to deal with and very customer oriented.
>
> Thanks, Karen Lahti
> Arizona Digestive Health
> Phoenix, AZ
>
>
> On Dec 27, 2011, at 12:58 PM, "Blazek, Linda" <lblazek <@t> digestivespecialists.com> wrote:
>
>> I have the Intellipath from BioCare. I love it. Service is spectacular. It's very user friendly. You do have to be consistent with your cleaning protocol. In the past 3-4 years I have only had 1 day of down time. The company is very proactive when it comes to service. You couldn't ask for better a better company to work with in both sales or service. If you want to contact me directly feel.
>> Linda Blazek
>> GI Pathology
>> 937 396-2623
>>
>> Sent from my iPhone
>>
>> On Dec 27, 2011, at 11:51 AM, "Cynthia Pyse" <cpyse <@t> x-celllab.com> wrote:
>>
>>> Hello Histonetters
>>>
>>> Happy Tuesday. I hope everyone had a Merry Christmas. It is amazing how
>>> times flies when you have time off. Now it's back to the old laboratory
>>> grind.
>>>
>>> We are in the market for a new immuno stainer. I currently use a Dako Link,
>>> which I am very pleased with. I have demoed the new Dako with great results,
>>> unfortunately we need 2 stainers and the pricing maybe a little out of our
>>> range.
>>>
>>> Is anyone using the Biocare Intellipath? What are the pro's and con's? How
>>> is their service? I currently use their detection system, Mach 4, but the
>>> majority of my antibodies are purchased from Dako. Any input on the daily
>>> use of the Intellipath from current users would be appreciated.
>>>
>>> Thanks in advance for the input
>>>
>>> Cindy
>>>
>>>
>>>
>>> Cindy Pyse, CLT, HT (ASCP)
>>>
>>> Laboratory Manager
>>>
>>> X-Cell Laboratories
>>>
>>> e-mail cpyse <@t> x-celllab.com
>>>
>>>
>>>
>>> _______________________________________________
>>> Histonet mailing list
>>> Histonet <@t> lists.utsouthwestern.edu
>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 5
> Date: Wed, 28 Dec 2011 14:21:44 +0000
> From: Nancy Schmitt <Nancy_Schmitt <@t> pa-ucl.com>
> Subject: [Histonet] gel pad for microtome vibration
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <906B4DA90ED1DB4DB6C7E94D7CEE6C367BC785 <@t> PEITHA.wad.pa-ucl.com>
> Content-Type: text/plain; charset="us-ascii"
>
> Good Morning-
> I am looking for some type of gel pad to place under the microtome (or perhaps under the waterbath...) to help with vibration - the water in the waterbath is jumping all over the place. Both instruments are new and the waterbath is much lighter in weight than the old ones.
> Thank you for your help with this!
> Nancy
> Dubuque, IA
>
>
>
>
> NOTICE: This email may contain legally privileged information. The information
> is for the use of only the intended recipient(s) even if addressed
> incorrectly. If you are not the intended recipient, please notify the sender
> that you have received it in error and then delete it along with any
> attachments. Thank you.
>
>
>
>
>
> ------------------------------
>
> Message: 6
> Date: Wed, 28 Dec 2011 08:34:07 -0600
> From: "Sharon Allen" <SAllen <@t> dsmanitoba.ca>
> Subject: [Histonet] FW: Quality Modules
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <BB6ADCD4B7ABB045A09A7634AC15CC610BEC824C <@t> hscxmsmx0010.ad.wrha.mb.ca>
> Content-Type: text/plain; charset="us-ascii"
>
> I am looking for information on Quality Modules that can be added into
> an existing IT system and system with great Quality Modules built into
> their IT platform.
>
> Thanks
>
>
>
> Sharon Allen
>
> Senior Medical Technologist
>
> Neuropathology Lab-MS435U
>
> Health Sciences Centre
>
> 820 Sherbrook Street
>
> Winnipeg,MB, CA
>
> R3A 1R9
>
> e-mail: sallen <@t> dsmanitoba.ca
>
>
>
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> This email and/or any documents in this transmission is intended for the
> addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.
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> Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original.
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> ------------------------------
>
> Message: 7
> Date: Wed, 28 Dec 2011 08:40:11 -0600
> From: "Sharon Allen" <SAllen <@t> dsmanitoba.ca>
> Subject: [Histonet] FW: standard for sm bx microtomy
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <BB6ADCD4B7ABB045A09A7634AC15CC610BEC824D <@t> hscxmsmx0010.ad.wrha.mb.ca>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi,
>
> Is there is a standard used for small biopsy Microtomy? I am wondering
> how other sites cut their biopsies (ie the number of sections per slide
> and the amount of roughing in between sections)? I am also curious
> about the number of slides per biopsy.
>
> Thanks for your help,
>
>
>
> Sharon Allen
>
> Senior Medical Technologist
>
> Neuropathology Lab-MS435U
>
> Health Sciences Centre
>
> 820 Sherbrook Street
>
> Winnipeg,MB, CA
>
> R3A 1R9
>
> e-mail: sallen <@t> dsmanitoba.ca
>
>
>
> -------------- next part --------------
> This email and/or any documents in this transmission is intended for the
> addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original.
>
> Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original.
>
> ------------------------------
>
> _______________________________________________
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 97, Issue 31
> ****************************************
>
>
>
> ------------------------------
>
> Message: 4
> Date: Thu, 29 Dec 2011 09:35:10 -0500
> From: "Jimmy Lofton" <loftonjt <@t> holycrosshealth.org>
> Subject: [Histonet] Shatter effect on specimens
> To: Histonet <@t> lists.utsouthwestern.edu
> Message-ID: <4EFC344E020000560000507C <@t> nodcdmg2.no.trinity-health.org>
> Content-Type: text/plain; charset=us-ascii
>
> Does anyone in histoland have corrected problems with shattered biopies, EM ect?
>
> Thanks for your help.
>
> Jimmy
>
>
> Jimmy Lofton, M.S., HT,CT(ASCP)
> Manager Histology Laboratory
> Holy Cross Hospital
> 1500 Forest Glen Road
> Silver Spring, MD 20910-1484
> 301-754-7353 (Phone)
> 301-754-8563 (Fax)
> loftonjt <@t> holycrosshealth.org
>
>
> Trinity Health MailGate made the following annotations
> ---------------------------------------------------------------------
> CONFIDENTIALITY NOTICE: This e-mail, including any attachments, is for the sole use of the intended recipient and may contain information that is confidential and privileged under state and Federal privacy laws. If you received this e-mail in error, be aware that any unauthorized use; disclosure, copying, or distribution is strictly prohibited. Please contact the sender immediately and destroy all copies of this message.
> ---------------------------------------------------------------------
>
>
>
> ------------------------------
>
> Message: 5
> Date: Thu, 29 Dec 2011 14:53:31 +0000
> From: "Marcum, Pamela A" <PAMarcum <@t> uams.edu>
> Subject: [Histonet] Histologist needed UAMS Little Rock AR
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <41D3A1AF6FEF0643BDC89E0516A6EA3220240815 <@t> Mail2Node2.ad.uams.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> Please no recruiters reply to this. We are a state University and we are not allowed to use your services. I am sorry however; this saves us both time.
>
> We currently have an opening for a registered HT or HTL at the University of Arkansas for Medical Sciences in Little Rock AR. It is a full time position with full benefits, vacation, sick time and 12 paid holidays per year. We are fast growing Histology Laboratory with new equipment and procedures coming for more experience with growth potential. The position is listed under UAMS Jobs as # 50006966. If you have any question you may call me at the number below. I will be happy to discuss the position and future plans with you.
>
> Best Regards,
>
> Pamela A Marcum
> AP Supervisor Histology
> Slot 502
> 4301 W Markham Street
> Little Rock AR 72205
> Office: 501-686-7554
> Fax: 501-686-7151
>
> Confidentiality Notice: This e-mail message, including any attachments,
> is for the sole use of the intended recipient(s) and may contain
> confidential and privileged information. Any unauthorized review,
> use, disclosure or distribution is prohibited. If you are not the
> intended recipient, please contact the sender by reply
> e-mail and destroy all copies of the original message..
>
>
> ------------------------------
>
> Message: 6
> Date: Thu, 29 Dec 2011 07:21:06 -0800
> From: "Paula Lucas" <plucas <@t> biopath.org>
> Subject: [Histonet] Saturday Coverage Orange County California
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <300217F9262C41D2A82C5951202C8995 <@t> biopath.local>
> Content-Type: text/plain; charset="us-ascii"
>
> We are in need of a per diem histotech who can work on Saturday mornings.
> We are located in Fountain Valley, California. Please send me an email if
> interested or fax resume to:
>
> 714 755-2984
>
> Thank you,
>
> Paula Lucas
>
>
>
> ------------------------------
>
> Message: 7
> Date: Thu, 29 Dec 2011 16:38:33 +0000
> From: joelle weaver <joelleweaver <@t> hotmail.com>
> Subject: RE: [Histonet] Shatter effect on specimens
> To: Jimmy Lofton <loftonjt <@t> holycrosshealth.org>
> Cc: Histonet <histonet <@t> lists.utsouthwestern.edu>,
> histonet-bounces <@t> lists.utsouthwestern.edu
> Message-ID: <SNT135-W60128325C5D463093F3F47D8AD0 <@t> phx.gbl>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> Chatter or shatter? Chatter to me is a microtomy problem more so, shatter I would look more to processing and handling... but people use those descriptions to mean both sometimes, but here are my thoughts #1 tissue type considerations - GI Bx and liver Bx especially- I have seen this alot when they get processed all together with other tissues( in a "do everything type of program"). #2- over dehydration, too prolonged or not enough gradation from dilute to absolute ETOH for certian tissue types( biopsies here) in the tissue processing program espeically those types in #1. #3 sometimes also more problems when rapid processing used with MW or when they had "rapid" processors with acetone espeically( gone now I think, the original Sakura), but the program just needs to be optimized if that is the case. #4 poor microtomy technique, and/or loose blade holder, block holder (due to rusted, worn out screws, or springs ) especially the little ones in the blade holder, or just not being clamped properly, or the angle can be off- this is the "chatter". Sometimes something is loose or worn, but some people clamp everything super-tight, and this is sometimes trouble too, or can't correct their angles. #5- I guess you would need to figure out what the source is intially to correct. But all could possibly be corrected with program changes to processing, microtome PM, training of microtomist to recognize the artifact on waterbath before it gets to the slide, and get more comfortable/confident with their microtome. At the bench I can correct the less than ideal processing sometimes when already "crunchy" by somewhat prolonged soaking in icy water before sectioning, and trying to section a little slower than I normally would. Can't help much with EM, not my experience, but I would guess some of the same issues, and/or vibration on the ultramicrotome?Joelle
>
> Joelle Weaver MAOM, (HTL) ASCP
>
> http://www.linkedin.com/in/joelleweaver
>
> > Date: Thu, 29 Dec 2011 09:35:10 -0500
>> From: loftonjt <@t> holycrosshealth.org
>> To: Histonet <@t> lists.utsouthwestern.edu
>> CC:
>> Subject: [Histonet] Shatter effect on specimens
>>
>> Does anyone in histoland have corrected problems with shattered biopies, EM ect?
>>
>> Thanks for your help.
>>
>> Jimmy
>>
>>
>> Jimmy Lofton, M.S., HT,CT(ASCP)
>> Manager Histology Laboratory
>> Holy Cross Hospital
>> 1500 Forest Glen Road
>> Silver Spring, MD 20910-1484
>> 301-754-7353 (Phone)
>> 301-754-8563 (Fax)
>> loftonjt <@t> holycrosshealth.org
>>
>>
>> Trinity Health MailGate made the following annotations
>> ---------------------------------------------------------------------
>> CONFIDENTIALITY NOTICE: This e-mail, including any attachments, is for the sole use of the intended recipient and may contain information that is confidential and privileged under state and Federal privacy laws. If you received this e-mail in error, be aware that any unauthorized use; disclosure, copying, or distribution is strictly prohibited. Please contact the sender immediately and destroy all copies of this message.
>> ---------------------------------------------------------------------
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 8
> Date: Thu, 29 Dec 2011 17:03:07 +0000
> From: Martha Ward-Pathology <mward <@t> wakehealth.edu>
> Subject: [Histonet] C4d immunofluorescent positive controls
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <B2CECB1B6665A4479056478F6DE3C4AB158618 <@t> EXCHDB3.medctr.ad.wfubmc.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> We are interested in finding out what type of positive control tissue everyone is using when performing C4d immunofluorescence on heart biopsies. Thanks in advance for any and all responses.
>
> Martha Ward, MT (ASCP) QIHC
> Manager, Molecular Diagnostics Lab
> Dept. of Pathology
> Wake Forest University Baptist Medical Center
> Winston-Salem, NC 27157
> 336-716-2104
>
>
>
> ------------------------------
>
> Message: 9
> Date: Thu, 29 Dec 2011 11:53:11 -0600
> From: Tunde Ajibade <tajibade <@t> echd.org>
> Subject: [Histonet] RE: C4d immunofluorescent positive controls
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <ABF9E8674BBE5448920911351AC6D3E50208FB5791 <@t> MCHMAIL.echd.org>
> Content-Type: text/plain; charset="us-ascii"
>
>
>
> Do you do renal transplant? If yes, look for renal biopsies submitted for immunofluorescence studies, Let the Pathologists confirm that the biopsy is acute cellular rejection from renal transplant, because acute cellular rejection from kidney transplant is known to be positive for c4d. After the studies, cut some extra frozen sections from this biopsy and fix in acetone for 20 mins, and cover the slide with parafilm to avoid cracking of slide and store in the freezer. They are good for some months.
>
> Tunde Ajibade BS, HTL(ASCP)QIHC
> Histology Supervisor
> Medical Center Hospital
> Odessa,TX
> Tel:432-640-2348
> Fax:432-640-2303
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology
> Sent: Thursday, December 29, 2011 11:03 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] C4d immunofluorescent positive controls
>
> We are interested in finding out what type of positive control tissue everyone is using when performing C4d immunofluorescence on heart biopsies. Thanks in advance for any and all responses.
>
> Martha Ward, MT (ASCP) QIHC
> Manager, Molecular Diagnostics Lab
> Dept. of Pathology
> Wake Forest University Baptist Medical Center
> Winston-Salem, NC 27157
> 336-716-2104
>
> _______________________________________________
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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>
>
> CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents.
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>
>
>
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