[Histonet] RE: C4d immunofluorescent positive controls

[Tunde Ajibade]

Do you do renal transplant? If yes, look for renal biopsies submitted for immunofluorescence studies, Let the Pathologists confirm that the biopsy is acute cellular rejection from renal transplant, because acute cellular rejection from kidney transplant is known to be positive for c4d. After the studies, cut some extra frozen sections from this biopsy and fix in acetone for 20 mins, and cover the slide with parafilm to avoid cracking of slide and store in the freezer. They are good for some months.

 Tunde Ajibade BS, HTL(ASCP)QIHC
 Histology Supervisor
 Medical Center Hospital
 Odessa,TX
 Tel:432-640-2348
 Fax:432-640-2303
 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology
Sent: Thursday, December 29, 2011 11:03 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] C4d immunofluorescent positive controls

We are interested in finding out what type of positive control tissue everyone is using when performing C4d immunofluorescence on heart biopsies.   Thanks in advance for any and all responses.

Martha Ward, MT (ASCP) QIHC
Manager, Molecular Diagnostics Lab
Dept. of Pathology
Wake Forest University Baptist Medical Center
Winston-Salem, NC 27157
336-716-2104

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