[Histonet] Re: Histonet Digest, Vol 97, Issue 30

Tue Dec 27 13:56:54 CST 2011

Hi Cindy,

What's the name of the new Dako stainer that you demo'd?

On Tue, Dec 27, 2011 at 1:00 PM,
<histonet-request <@t> lists.utsouthwestern.edu>wrote:

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> Today's Topics:
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>   1. RE: HSV1 and HSV2 (Hoekert, W.E.J.)
>   2. RELIA HOT Histology Job Alert. 12/27/2011 Great   Opportunity
>      for a histology tech in Virginia. (Pam Barker)
>   3. immuno stainers (Cynthia Pyse)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Tue, 27 Dec 2011 10:38:20 +0100
> From: "Hoekert, W.E.J." <W.E.J.Hoekert <@t> olvg.nl>
> Subject: RE: [Histonet] HSV1 and HSV2
> To: "Evans, Andria B" <aevans3 <@t> lghealth.org>,
>        <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <1190CB05C44B13409483514729C2FC3601F84207 <@t> PAIT42.olvg.nl>
> Content-Type: text/plain;       charset="iso-8859-1"
>
> For the immunologic pAB cocktail we are using Protease 1 (4 min) and ab
> incubation of 32 min. The only problem is that mast cells are also
> staining, it is a side effect of the protease 1.
>
> (AB concentration 1:400)
>
> Willem Hoekert
> OLVG Amsterdam
> The Netherlands
>
>
>
>
> ________________________________
>
> Van: histonet-bounces <@t> lists.utsouthwestern.edu namens Evans, Andria B
> Verzonden: vr 23-12-2011 18:48
> Aan: histonet <@t> lists.utsouthwestern.edu
> Onderwerp: [Histonet] HSV1 and HSV2
>
>
>
> I am currently having issues with our HSV1 and HSV2 staining tissue
> components that it shouldn't be/background.  We are running Ventana
> Benchmark XTs and Ultras.  Using Dako's polyclonal rabbit concentrate.
>  Does anyone out there in histoland have a concentration with a protocol
> that works and looks clean?
>
> Thank you for your help!
>
> Andria B Evans HTL(ASCP)CM
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>
> ------------------------------
>
> Message: 2
> Date: Tue, 27 Dec 2011 11:26:52 -0500
> From: "Pam Barker" <relia1 <@t> earthlink.net>
> Subject: [Histonet] RELIA HOT Histology Job Alert. 12/27/2011 Great
>        Opportunity for a histology tech in Virginia.
> To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <2AD338F5D60F42969C3852F762D09410 <@t> ownerf1abaad51>
> Content-Type: text/plain;       charset="iso-8859-1"
>
> Hi Histonetters,
> Seasons Greetings!!
> I hope everyone enjoyed a wonderful holiday weekend.  I have a brand new
> histology job that I am pretty excited about and I wanted to take a
> minute and share the info with you:
>
> RELIA Solutions the nation’s only recruiting firm dedicated to the
> nationwide permanent placement of histology professionals is assisting a
> leading lab in Roanoke, VA in their search for a histology tech.  ASCP
> HT/HTL and 3 years experience preferred.  IHC is a plus.  This is a day
> shift (early morning) M-F full time permanent position.  My client
> offers a great salary and excellent benefits.  For more information
> please contact Pam Barker at  <mailto:relia1 <@t> earthlink.net>
> relia1 <@t> earthlink.net or toll free at 866-607-3542
>
> If you or anyone you know might be interested in hearing more about this
> opportunity please contact me.  I can be reached at
> <mailto:relia1 <@t> earthlink.net> relia1 <@t> earthlink.net or toll free at
> 866-607-3542.
>
> Thanks-Pam
>
>
> Thank You!
>
>
>
> Pam Barker
> President
> RELIA
> Specialists in Allied Healthcare Recruiting
> 5703 Red Bug Lake Road #330
> Winter Springs, FL 32708-4969
> Phone: (407)657-2027
> Cell:     (407)353-5070
> FAX:     (407)678-2788
> E-mail:  <mailto:relia1 <@t> earthlink.net> relia1 <@t> earthlink.net
>  <http://www.facebook.comPamBarkerRELIA> www.facebook.comPamBarkerRELIA
>  <http://www.linkedin.com/reliasolutions>
> www.linkedin.com/reliasolutions
>  <http://www.myspace.com/pamatrelia> www.myspace.com/pamatrelia
>  <http://www.twitter.com/pamatrelia> www.twitter.com/pamatrelia
>
>
>
> ------------------------------
>
> Message: 3
> Date: Tue, 27 Dec 2011 11:49:50 -0500
> From: "Cynthia Pyse" <cpyse <@t> x-celllab.com>
> Subject: [Histonet] immuno stainers
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <003e01ccc4b7$8cb51a40$a61f4ec0$@com>
> Content-Type: text/plain;       charset="us-ascii"
>
> Hello Histonetters
>
> Happy Tuesday. I hope everyone had a Merry Christmas. It is amazing how
> times flies when you have time off. Now it's back to the old laboratory
> grind.
>
> We are in the market for a new immuno stainer. I currently use a Dako Link,
> which I am very pleased with. I have demoed the new Dako with great
> results,
> unfortunately we need 2 stainers and the pricing maybe a little out of our
> range.
>
> Is anyone using the Biocare Intellipath? What are the pro's and con's? How
> is their service? I currently use their detection system, Mach 4, but the
> majority of my antibodies are purchased from Dako. Any input on the daily
> use of the Intellipath from current users would be appreciated.
>
> Thanks in advance for the input
>
> Cindy
>
>
>
> Cindy Pyse, CLT, HT (ASCP)
>
> Laboratory Manager
>
> X-Cell Laboratories
>
> e-mail cpyse <@t> x-celllab.com
>
>
>
>
>
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> End of Histonet Digest, Vol 97, Issue 30
> ****************************************
>