[Histonet] HSV1 and HSV2

[Hoekert, W.E.J.]

For the immunologic pAB cocktail we are using Protease 1 (4 min) and ab incubation of 32 min. The only problem is that mast cells are also staining, it is a side effect of the protease 1. 
 
(AB concentration 1:400)
 
Willem Hoekert
OLVG Amsterdam
The Netherlands
 
 
 

________________________________

Van: histonet-bounces <@t> lists.utsouthwestern.edu namens Evans, Andria B
Verzonden: vr 23-12-2011 18:48
Aan: histonet <@t> lists.utsouthwestern.edu
Onderwerp: [Histonet] HSV1 and HSV2



I am currently having issues with our HSV1 and HSV2 staining tissue components that it shouldn't be/background.  We are running Ventana Benchmark XTs and Ultras.  Using Dako's polyclonal rabbit concentrate.  Does anyone out there in histoland have a concentration with a protocol that works and looks clean?

Thank you for your help!

Andria B Evans HTL(ASCP)CM
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